Elucidation of the cell death mechanism after the boron-neutron capture therapy in oral cancer

• Project/Area Number: 25861967
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Nara Medical University
• Principal Investigator: Yamakawa Nobuhiro 奈良県立医科大学, 医学部, 助教 (00526709)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: BNCT / apoptosis / p53 / アポトーシス

Outline of Final Research Achievements

The boron-neutron capture therapy(BNCT) is one of the high LET radiation treatment.As for the high LET radiation, high effect of treatment is obtained regardless of the genotype of the cancer.A purpose of this study is to determine a control structure of the cell death of the cancer cell by BNCT.We used human gingival squamous cell carcinoma cells Ca9-22 for a study.Cellular sensitivities were determined using colony-forming assays. Apoptosis was detected and quantified with Hoechst 33342 staining. The activity of caspase-3 and PARP were analyzed with Western blotting.
During study period, our study was adopted in Kyoto University Research Reactor Institute.A nuclear reactor did not work after Great East Japan Earthquake.Therefore our study is only a study with the low LET radiation.