Continuous monitoring of caspase-3 activation induced by propofol in developing mouse brain
| Project/Area Number |
25861974
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Showa University |
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Principal Investigator |
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| Research Collaborator |
KONNO Ayumi
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | カスパーゼ3 / SCAT3 / FRET / プロポフォール / アポトーシス / 脳神経細胞死 / 全身麻酔薬 / カスパーゼ３ |
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| Outline of Final Research Achievements |
The neurotoxicity of anesthetics on the developing brain has drawn the attention of anesthesiologists. Previous studies have shown that prolonged exposure to anesthetics causes apoptosis. Exactly when the apoptotic cascade starts in the brain remains uncertain. We describe the development of a continuous monitoring system to detect caspase-3 activation using an in vivo model. Brain slices from neonatal SCAT3 transgenic mice with a heterozygous genotype (n = 20) were used for the monitoring of caspase-3 cleavage. SCAT3 is a fusion protein of ECFP and Venus connected by a caspase-3 cleavable peptide, DEVD. A specimen from the hippocampal CA1 sector was mounted on a confocal laser microscope and was continuously superfused with propofol. We observed a shift in the histogram toward the right over time, indicating caspase-3 activation at five hours in the propofol. Thus, real-time FRET imaging was capable of identifying the onset of apoptosis triggered by propofol in neonatal brain slices.
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Report
(4 results)
Research Products
(1 results)
