| Project/Area Number |
25861998
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
KERA Yohei 東北大学, 大学病院, 助教 (90647950)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | メカニカルストレス / 伸展 / エピジェネティクス / 歯根膜細胞 / 骨芽細胞 |
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| Outline of Final Research Achievements |
Extension among mechanical stress causes differentiation of periodontal ligament cell into osteoblast, therfore, it causes bone formation. Because we tried to begin the extension experiment using silicon chamber, we investigated whether this experimental system was established. We extended mouse osteoblast-like cells (MC3T3-E1) using the silicon chamber, and investigated cyclooxygenase-2 (COX2) gene expression which was an indicator of extension and involved in bone formation. COX2 gene expression in extension group was higher than control , so experimental system was established.
It was reported that COX2 was suppressed by methionine adenosyltransferase II (MATII) in mouse fibroblasts. When the same experiment was performed in MC3T3-E1, it was similar results. It was suggested that extension stress caused bone formation through MATII and COX2.
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