Microarray-based analyses of the mechanisms of tooth morphogenesis regulated by IGF-I

• Project/Area Number: 25861999
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: CHIDA Toko 東北大学, 大学病院, 助教 (40647947)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 再生医療 / IGF-1 / 歯の形態 / マイクロアレイ

Outline of Final Research Achievements

We identified the molecules which are regulated by IGF-I with microarray analysis to clarify the mechanisms of tooth morphogenesis regulated by IGF-I. As a result, IGF-I upregulated expressions of various molecules that modulate tooth development. Moreover, we performed in vivo study to analyze the effects of IGF-I on mice dental mesenchymal cells. IGF-I induced proliferation and differentiation of dental mesenchymal cells.

Research Products

• [Journal Article] The expression of Fn14 via mechanical stress-activated JNK contributes to apoptosis induction in osteoblasts. (2014)
  • Author(s): Matsui H, Fukuno N, Kanda Y, Kantoh Y, Chida T, Nagaura Y, Suzuki O, Nishitoh H, Takeda K, Ichijo H, Sawada Y, Sasaki K, Kobayashi T, Tamura S.
  • Journal Title: J Biol Chem Volume: 289 Pages: 6438-50
  • Peer Reviewed / Open Access
• [Journal Article] The expression of Fn14 via mechanical stress-activated JNK contributes to apoptosis induction in osteoblasts (2014)
  • Author(s): Matsui H, Fukuno N, Kanda Y, Kantoh Y, Chida T, Nagaura Y, Suzuki O, Nishitoh H, Takeda K. Ichijo H, Sawada Y, Sasaki K, Kobayashi T and Tamura S
  • Journal Title: The Journal of Biological Chemistry Volume: in press
  • Peer Reviewed
• [Presentation] N-Myristoylation is essential for protein phosphatases PPM1A and PPM1B to dephosphorylate their physiological substrates in cells (2013)
  • Author(s): Takayasu Kobayashi, Toko Chida, Masakatsu Ando, Tasuku Matsuki, Yutaro Masu, Yuko Nagaura, Shinri Tamura
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜