| Project/Area Number |
25862013
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
TAKASHIMA Yukiko 岡山大学, 医歯(薬)学総合研究科, 助教 (30589768)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | Streptococcus mutans / グルカン結合タンパク / 齲蝕 / バイオインフォマティクス / う蝕 / gbpC 遺伝子 / バイオフィルム / 結合ドメイン / デキストラン結合能 / バイオインフォマティックス / Gbp |
|---|
| Outline of Final Research Achievements |
Streptococcus mutans produces glucan-binding proteins (Gbps), which participate in biofilm development. Among those, GbpC encoded by the gbpC is known to possess a cariogenic protein. We examined the dextran-binding domain of GbpC using bioinformatics analysis. First, truncated recombinant GbpC (rGbpC) including the upstream or downstream region of the gbpC were produced, and dextran-binding ability was examined. The rate of dextran-binding of truncated rGbpC including the upstream region was higher than that including the downstream region. Next, 5 regions were specified as possible molecular binding sites using bioinformatics analysis and individual mutant strains with deletion of each region were generated, dextran-binding was again examined. The rate of dextran-binding for the mutant strain deficient of the center region was significantly lower as compared to the others. These results suggest that the dextran-binding domain may be located in the center of the gbpC.
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