| Project/Area Number |
25862020
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
OHARA YUKARI 広島大学, 医歯薬保健学研究院(歯), 助教 (80634469)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 小児齲蝕 / Streptococcus mutans / streptococcus mutans / バイオフィルム / 耐酸性能 |
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| Outline of Final Research Achievements |
In thie study, clinical isolated Streptococcus mutans (S. mutans) were used. Biofilm samples were cultivated at 37℃ in three-channel flow cells with a flow of Brain Heart Infusion(BHI). After extracted RNA from biofilm samples, the expression levels of gtfB and atpD that dental caries related genes were quantitated by real-mime RT-PCR. Compare with caries high risk group and low risk group, the expression levels of gtfB and atpD were increased at pH5.5 of BHI than pH7.0. And the increase level of caries high risk group was higher than low risk group. These results suggested that the severity of dental caries is related the ability of S. mutans to adapt the acidic environment.
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