| Project/Area Number |
25862034
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
ITO Tatsuro 日本大学, 松戸歯学部, 助教 (60635126)
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| Research Collaborator |
SENPUKU Hidenobu
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 小児歯科学 / バイオフィルム / SspBペプチド |
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| Outline of Final Research Achievements |
The binding of S. mutans to the salivary pellicle is a key event in oral biofilm (B.F.) formation. In addition, B.F. is a reservoir of drug resistance genes. Hence, the establishment of a new biofilm control method, which differs from the conventional bactericidal or bacteriostatic approaches, has been desired.
(1) The binding activity of the SspB peptides to saliva was detected by ELISA. In the present study, SspB (A4K-A11K) peptide showed the highest level of binding to saliva. A4K-A11K inhibited adherence of S. mutans to saliva; however, the peptide did not affected oral commensal. (2) To determine if A4K-A11K has anti-microbial activity, A4K-A11K and 0.04% chlorhexidine (CHX) were applied into S. mutans cultures. CHX inhibited growth of S. mutans; whereas the A4K-A11K did not show growth inhibition. (3) A4K-A11K inhibited B.F. formation of S. mutans on the saliva-coated polystyrene plates.
A4K-A11K is available to regulate dental biofilm-dependent diseases such as caries.
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