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Establishment of pathogenic oral biofilm regulation approach

Research Project

Project/Area Number 25862034
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Research InstitutionNihon University

Principal Investigator

ITO Tatsuro  日本大学, 松戸歯学部, 助教 (60635126)

Research Collaborator SENPUKU Hidenobu  
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords小児歯科学 / バイオフィルム / SspBペプチド
Outline of Final Research Achievements

The binding of S. mutans to the salivary pellicle is a key event in oral biofilm (B.F.) formation. In addition, B.F. is a reservoir of drug resistance genes. Hence, the establishment of a new biofilm control method, which differs from the conventional bactericidal or bacteriostatic approaches, has been desired.
(1) The binding activity of the SspB peptides to saliva was detected by ELISA. In the present study, SspB (A4K-A11K) peptide showed the highest level of binding to saliva. A4K-A11K inhibited adherence of S. mutans to saliva; however, the peptide did not affected oral commensal. (2) To determine if A4K-A11K has anti-microbial activity, A4K-A11K and 0.04% chlorhexidine (CHX) were applied into S. mutans cultures. CHX inhibited growth of S. mutans; whereas the A4K-A11K did not show growth inhibition. (3) A4K-A11K inhibited B.F. formation of S. mutans on the saliva-coated polystyrene plates.
A4K-A11K is available to regulate dental biofilm-dependent diseases such as caries.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2015 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (9 results)

  • [Journal Article] A novel animal model for dry mouth: E2f1-deficient NOD/SCID mice.2014

    • Author(s)
      Satoh K, Narita T, Matsuki-Fukushima M, Okabayashi K, Yamazaki F, Arai T, Ito T, Senpuku H, Sugiya H.
    • Journal Title

      J Oral Biosci

      Volume: 56 Issue: 1 Pages: 18-22

    • DOI

      10.1016/j.job.2013.08.004

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Establishment of SspB peptide binding assay for Porphyromonas gingivalis colonization processes2015

    • Author(s)
      Tatsuro Ito, Takahiro Ichinosawa, Nana Ikematsu-Ito, Takehiko Shimizu
    • Organizer
      IAPD 2015 Conference
    • Place of Presentation
      Glasgow, United Kingdom
    • Year and Date
      2015-07-01 – 2015-07-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] Treatment of eruption disturbance of permanent successor after trauma to primary tooth: 2 cases reports2015

    • Author(s)
      Nana Ikematsu-Ito, Tatsuro Ito, Kanako Sawamoto, Rina Shimada, Takehiko Shimizu
    • Organizer
      IAPD 2015 Conference
    • Place of Presentation
      Glasgow, United Kingdom
    • Year and Date
      2015-07-01 – 2015-07-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] SspBペプチドによるStreptococcus mutans初期付着阻害効果の検討2015

    • Author(s)
      市野澤 隆宏, 倉田 香, 倉田 康弘, 伊藤 龍朗, 清水 武彦
    • Organizer
      第53回日本小児歯科学会大会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2015-05-21 – 2015-05-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 歯周病リスク診断用ペプチド結合アッセイの確立2015

    • Author(s)
      伊藤 龍朗, 市野澤 隆宏, 荒井 延子, 伊藤 (池松) 奈々, 清水 武彦
    • Organizer
      第53回日本小児歯科学会大会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2015-05-21 – 2015-05-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 下顎に生じたエナメル上皮腫に対して架橋プレートを適用し顎骨切除を回避した1例2014

    • Author(s)
      岩井 聡, 伊藤 耕, 高橋 康輔, 青木 暁宣, 伊藤 奈々, 伊藤 龍朗, 清水 武彦, 近藤 壽郎
    • Organizer
      第26回日本小児口腔外科学会総会・学術大会
    • Place of Presentation
      栃木県総合文化センター
    • Year and Date
      2014-11-07 – 2014-11-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] Contribution of analogous SspB peptide to saliva-mediated Porphyromonas gingivalis attachment2014

    • Author(s)
      Tatsuro Ito, Hidenobu Senpuku, Takahiro Ichinosawa, Takehiko Shimizu,Takahide Maeda
    • Organizer
      92nd IADR GENERAL SESSION
    • Place of Presentation
      Cape Town, South Africa
    • Year and Date
      2014-06-25 – 2014-06-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] SspB由来ペプチドによるPorphyromonas gingivalis 定着メカニズムの検討2014

    • Author(s)
      伊藤 龍朗,市野澤 隆宏,清水 武彦,前田 隆秀
    • Organizer
      第52回日本小児歯科学会大会
    • Place of Presentation
      きゅりあん(品川区立総合区民会館)
    • Year and Date
      2014-05-16 – 2014-05-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Contribution of Analogous SspB peptide to Saliva-mediated Porphyromonas gingivalis2014

    • Author(s)
      Tatsuro Ito, Hidenobu Senpuku, Takahiro Ichinosawa, Takehiko Shimizu, Takahide Maeda
    • Organizer
      92nd IADR General Session
    • Place of Presentation
      Cape Town, South Africa
    • Related Report
      2013 Research-status Report
  • [Presentation] Porphyromonas gingivalisの定着メカニズムに関する基礎的検討

    • Author(s)
      伊藤 龍朗, 前田 隆秀
    • Organizer
      第28回 日本小児歯科学会大会 関東地方会
    • Place of Presentation
      神奈川歯科大学 横須賀キャンパス
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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