Analysis of involved specific transcription factor to differentiation Induction at periodontal tissue component cells

• Project/Area Number: 25862057
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontology
• Research Institution: Nihon University
• Principal Investigator: HIDEKI Takai 日本大学, 松戸歯学部, 助教 (30453898)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 転写因子

Outline of Final Research Achievements

Establishment of periodontal tissue regeneration therapy is important to understand the biological properties of the periodontal tissue component cells. We was searched what kinds of phenotype cells is HPDL induced to differentiation by knocking down the specifically expressed transcription factors at periodontal tissue. Result of knock down specifically expressed Transcription factor at periodontal tissue (KLF12, Twist2 and Pax9), Sox5 mRNA expression level is increased, Sp7mRNA expression level was decreased. we suggested that HPDL could be induced to differentiate into chondrocytes.