A role of amelotin in inflamed gingival junctional epithelium

• Project/Area Number: 25862059
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontology
• Research Institution: Nihon University
• Principal Investigator: NAKAYAMA Yohei 日本大学, 松戸歯学部, 講師 (30434088)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: アメロチン / 歯周組織 / 歯肉付着上皮 / 慢性歯周炎 / エナメルタンパク質 / 付着上皮 / 転写調節

Outline of Final Research Achievements

Amelotin (Amtn) is secreted by ameloblasts at maturation stage of enamel formation, the expression persists at junctional epithelium after tooth eruption. Therefore it thought that Amtn might be associated with the homeostasis of periodontal tissue. In this study, we investigated the change of Amtn expression in inflammation using human gingival tissue and human fibroblasts (HGFs) from patients with periodontitis or healthy.
In the inflamed gingival tissue from patients with periodontitis, Amtn RNA expression levels were increased and Amtn localization was slightly spread compared with healthy gingiva. Moreover Amtn RNA levels and transcriptional activity of Amtn gene were increased by proinflammatory cytokines in HGFs. Thus they indicated that Amtn might play a role at junctional epithelium in inflammation.

Research Products

• [Journal Article] Enamel Hypomineralization and Structural Defects in Amelotin-deficient Mice. (2015)
  • Author(s): Nakayama Y, Holcroft J, Ganss B.
  • Journal Title: J Dent Res. Volume: Feb.24 Issue: 5 Pages: 1-9
  • DOI: 10.1177/0022034514566214
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Proinflammatory cytokines induce amelotin transcription in human gingival fibroblasts. (2014)
  • Author(s): Nakayama Y, Takai H, Matsui S, Matsumura H, Zhou L, Kato A, Ganss B, Ogata Y.
  • Journal Title: J Oral Sci. Volume: 56 Pages: 261-268
  • NAID: 130004705235
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transcriptional regulation of amelotin gene by proinflammatory cytokines in gingival fibroblasts. (2014)
  • Author(s): Nakayama Y, Takai H, Matsui S, Zhou L, Abiko Y, Ganss B, Ogata Y.
  • Journal Title: Connect Tissue Res. Volume: 55(S1) Pages: 18-20
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Transcriptional regulation of amelotin gene by proinflammatory cytokines in gingival fibroblasts (2013)
  • Author(s): Yohei Nakayama, Hideki Takai, Sari Matsui, Liming Zhou, Hiroyoshi Matsumura, Ayako Kato, Yoshimitsu Abiko, Bernhard Ganss, Yorimasa Ogata
  • Organizer: 11th INTERNATIONAL CONFERENCE ON THE CHEMISTRY AND BIOLOGY OF MINERALIZED TISSUES
  • Place of Presentation: Lake Geneva, Wisconsin, USA