Isolation and characterization of the human alveolar bones osteoblast for bone regeneration therapy
| Project/Area Number |
25862067
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontology
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
Makoto Aino 愛知学院大学, 歯学部, 助教 (20572811)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 歯槽骨 / 再生 / 骨再生 / 歯周病 / 再生治療 / 骨芽細胞 / 歯槽骨再生 / 中高齢者 |
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| Outline of Final Research Achievements |
Establishment of human osteoblast cultures that retain bone forming capacity is one of the prerequisites for successful bone regeneration therapy. Because osteoblasts harvested from adults exhibit limited growth, the use of immature osteoblasts that can expand ex vivo should greatly facilitate bone regeneration therapy. In this study, we developed immature human osteoblasts isolated from aged alveolar bone (HAOBs). HAOBs obtained after the collagenase digestion of alveolar bones from elderly donors. Then, we assessed osteogenic ability of HAOB transplantation into immunodeficient mice. And, we performed global gene expression analysis to identify functional marker for HAOB. HAOBs, which can differentiate into osteoblasts and have a robust bone-forming ability, were successfully extracted from aged donors. We found that the HAOBs exhibited a higher osteogenic ability compared with those of human mesenchymal stem cells and highly expressed NEBULETTE (NEBL) with osteogenic abilities.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Five-year clinical results for treatment of intrabony defects with EMD, guided tissue regeneration and open-flap debridement: a case series2014
Author(s)
Mitani A,Takasu H,Horibe T, Furuta H, Nagasaka T, Aino M, Fukuda M, Fujimura T, Mogi M, Noguchi T.
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Journal Title
J Periodont Res
Volume: 印刷中
Issue: 1
Pages: 123-130
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Isolation and characterization of the human immature osteoblast culture system from the alveolar bones of aged donors for bone regeneration therapy.2014
Author(s)
Aino M, Nishida E, Fujieda Y, Orimoto A, Mitani A, Noguchi T, Makino H, Murakami S, Umezawa A, Yoneda T, Saito M.
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Journal Title
Expert Opin Biol Ther.
Volume: 12
Pages: 1731-44
Related Report
Peer Reviewed
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[Journal Article] Irradiation with a low-level diode laser induces the developmental endothelial locus-1 gene and reduces pro-inflammatory cytokines in epithelial cells2014
Author(s)
Fujimura T, Mitani A, Fukuda M, Mogi M, Osawa K, Takahashi S, Aino M, Iwamura Y, Miyajima S, Yamamoto H, Noguchi T
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Journal Title
Lasers Med Sci
Volume: 29
Issue: 3
Pages: 987-994
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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