| Project/Area Number |
25870014
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
Molecular biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
NAGAO Koji 北海道大学, 先端生命科学研究科(研究院), 講師 (60426575)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | エピジェネティクス / ヘテロクロマチン / ポリコーム複合体 |
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| Outline of Final Research Achievements |
Polycomb repressive complex 2 (PRC2) play a key role in silencing developmentally regulated genes to establish and maintain facultative heterochromatin. The core PRC2 complex comprises four components (EZH1/2, SUZ12, EED and RBBP4/7) and methylates histone H3 at Lys 27 through its enzymatic subunit EZH1/2. In addition, five regulatory components have been identified in mammalian PRC2. However, how mammalian PRC2 regulates various genomic sites and collaborates with other chromatin modulators remains unclear. To define the entire composition of PRC2 in human, we performed a comprehensive proteomic analysis of all PRC2 components using by semi-quantitative mass-spectrometry. We established the protein interaction network between PRC2 components and found that distinct PRC2 complexes are formed in human cells.
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