Structural analysis of HLA-G6 isoform bound to LILRB2 receptor

Research Project

Project/Area Number	25870019
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Structural biochemistry Functional biochemistry
Research Institution	Hokkaido University
Principal Investigator	KUROKI KIMIKO 北海道大学, 薬学研究科(研究院), 助教 (90553313)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	HLA-G6 / 電子顕微鏡解析 / 表面プラスモン共鳴法 / CIAマウス / LILR / PIR-B / 抗炎症効果 / 相互作用解析
Outline of Final Research Achievements	I performed structural and functional analysis of the HLA-G6 isoform. Electron microscopy technique revealed the three-dimensional structure of HLA-G2/G6 homodimer which resemble HLA class I and HLA class II heterodimer molecule. In addition, I examined the function of HLA-G6 isoform in CIA mice. HLA-G6 specifically bound to the mouse receptor, PIR-B, and showed significant anti-inflammatory effects in vivo.

Report

(3 results)