Development of Cell Delivery Carrier Consisting of Free-standing Polymer Nanosheets
Project/Area Number |
25870050
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Biomedical engineering/Biomaterial science and engineering
Polymer chemistry
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Research Institution | Waseda University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Keywords | ナノバイオ材料 / 生体材料科学 / 生体組織工学 / 再生医療 / 細胞移植 / 加齢黄斑変性症 / 高分子超薄膜 / 微細加工技術 / 生体材料 / 細胞移植療法 / 網膜色素上皮細胞 / 高分子ナノ薄膜 / ナノシート / 微細加工 / 移植・再生医療 / ナノバイオ / 細胞デリバリー |
Outline of Final Research Achievements |
For the treatment of intractable retinal diseases such as age-related macular degeneration, in this study we envisioned development of a cell delivery carrier using flexible polymer nanosheets to transplant retinal pigment epithelial cells into a narrow subretinal place. Micropatterned nanosheets were fabricated from poly(lactic-co-glycolic acid), on which the cells were selectively cultured. It was clarified that the engineered cell monolayer on the micropatterned nanosheet was aspirated and injected through a clinical needle without loss of cell viability (approximately 90% viable). The cell monolayer was also transplanted to the subretinal place of rats near the macula. Overall, for the first time we demonstrated the utility of nanosheets in cell delivery system.
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Report
(3 results)
Research Products
(53 results)
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[Journal Article] Spatial coordination of cell orientation directed by unique nanoribbon sheets.2015
Author(s)
Fujie, T., Shi, X., Ostrovidov, S., Liang, X., Nakajima, K., Chen, Y., Wu, H., Khademhosseini, A.
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Journal Title
Biomaterials
Volume: 53
Pages: 86-94
DOI
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Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Three-dimensional co-culture of C2C12/PC12 cells improves skeletal muscle tissue formation and function.2014
Author(s)
Ostrovidov, S., Ahadian, S., Ramon-Azcon, J., Hosseini, V., Fujie, T., Prakash Parthiban, S., Shiku, H., Matsue, T., Kaji, H., Ramalingam, M., Bae, H., Khademhosseini, A.
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Journal Title
Journal of Tissue Engineering and Regenerative Medicine
Volume: -
Issue: 2
Pages: 582-595
DOI
Related Report
Peer Reviewed
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[Journal Article] Myotube formation on gelatin nanofibers - multiwalled carbon nanotubes hybrid scaffolds.2014
Author(s)
Ostrovidov, S., Shi, X., Zhang, L., Liang, X., Kim, S-B., Fujie, T., Ramalingam, M., Chen, M., Nakajima, K., Al-Hazmi, F., Bae, H., Memic, A., Khademhosseini, A.
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Journal Title
Biomaterials
Volume: 35
Issue: 24
Pages: 6268-6277
DOI
Related Report
Peer Reviewed
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[Journal Article] Periosteum-mimetic structures made from freestanding microgrooved nanosheets.2014
Author(s)
Shi, X., Fujie, T., Saito, A., Takeoka, S., Hou, Y., Shu, Y., Chen, M., Wu, H., Khademhosseini, A.
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Journal Title
Advanced Materials
Volume: 26
Issue: 20
Pages: 3290-3296
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Micropatterned polymeric nanosheets for local delivery of an engineered epithelial monolayer2014
Author(s)
Fujie, T., Mori, Y., Ito, S., Nishizawa, M., Bae, H., Nagai, N., Onami, H., Abe, T., Khademhosseini, A., Kaji, H.
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Journal Title
Advanced Materials
Volume: 26
Issue: 11
Pages: 1699-1705
DOI
Related Report
Peer Reviewed
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[Journal Article] Engineered nanomembranes for directing cellular organization towards flexible biodevices2013
Author(s)
Fujie, T., Ahadian, S., Liu, H., Chang, H., Ostrovidov, S., Wu, H., Bae, H., Nakajima, K., Kaji, H., Khademhosseini, A.
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Journal Title
Nano Letters
Volume: 13
Issue: 8
Pages: 3185-3192
DOI
Related Report
Peer Reviewed
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[Presentation] Development of freestanding microgrooved nanosheets with periosteum-mimetic structure.2014
Author(s)
Fujie, T., Shi, X., Ostrovidov, S., Wu, H., Khademhosseini, H., Takeoka, S.
Organizer
Materials Today Asia 2014
Place of Presentation
HongKong
Year and Date
2014-12-09 – 2014-12-12
Related Report
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[Presentation] 骨膜様微細溝構造を有する高分子ナノシートの開発2014
Author(s)
藤枝 俊宣, Shi Xuetao, Ostrovidov Serge, 武岡 真司, Khademhosseini Ali.
Organizer
第36回日本バイオマテリアル学会大会
Place of Presentation
東京
Year and Date
2014-11-17 – 2014-11-18
Related Report
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[Presentation] Fabrication of Micropatterned Polymeric Nanosheets for Local Delivery of an Engineered Epithelial Monolayer.2014
Author(s)
Fujie, T., Mori, Y., Nishizawa, M., Nagai, N., Abe, T., Khademhosseini, A., Kaji, H.
Organizer
JSPS A3 Foresight International Symposium on Nano-Biomaterials and Regenerative Medicine
Place of Presentation
Tokyo
Year and Date
2014-10-08 – 2014-10-09
Related Report
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[Presentation] Micropatterned polymeric nanosheets for local injection of an engineered epithelial monolayer.2014
Author(s)
Fujie, T., Mori, Y., Nishizawa, M., Nagai, N., Abe, T., Khademhosseini, A., Kaji, H.
Organizer
248th ACS National Meeting & Exposition
Place of Presentation
San Francisco
Year and Date
2014-08-10 – 2014-08-14
Related Report
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[Presentation] Injectable Micropatterned Polymeric Nanosheets for Local Delivery of an Engineered Epithelial Monolayer.2014
Author(s)
Fujie, T., Mori, Y., Nishizawa, M., Nagai, N., Abe, T., Khademhosseini, A., Kaji, H.
Organizer
2014 MRS Spring Meeting & Exhibit
Place of Presentation
San Francisco
Year and Date
2014-04-21 – 2014-04-25
Related Report
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[Book] Bioinspired Muscle Tissue Devices (ch. 35), in Handbook of Biomimetics and Bioinspiration2014
Author(s)
Fujie, T., Ostrovidov, S., Ahadian, S., S. Prakash Parthiban, Khademhosseini, A., Kaji, H
Total Pages
16
Publisher
World Scientific Publishing
Related Report
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[Patent(Industrial Property Rights)] 形状制御されたナノシート及びその製造方法2015
Inventor(s)
岩瀬英治,新保創太,武岡真司,藤枝俊宣,梶 弘和,阿部俊明
Industrial Property Rights Holder
岩瀬英治,新保創太,武岡真司,藤枝俊宣,梶 弘和,阿部俊明
Industrial Property Rights Type
特許
Industrial Property Number
2015-043990
Filing Date
2015-03-05
Related Report
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