| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
Amphibians have high regenerative capacity. For example, they can regenerate a limb after amputation. However, difficulty in manipulating gene expression in spatially restricted manner has so far hindered elucidation of the molecular mechanisms of regeneration in amphibians. In this study, I applied two types of gene induction systems, IR-LEGO and temperature stimulator to amphibians and enabled local gene induction. As a result, function of a specific gene has been revealed by single cell level gene induction by IR-LEGO. Furthermore, gene induction exclusively in a specific region in a limb has been done by using temperature stimulator.
|
