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Local gene induction system in an amphibian and its application to morphogenetic regeneration

Research Project

Project/Area Number 25870058
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Developmental biology
Morphology/Structure
Research InstitutionHirosaki University (2014-2016)
Tohoku University (2013)

Principal Investigator

Yokoyama Hitoshi  弘前大学, 農学生命科学部, 准教授 (90455816)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords再生 / 両生類 / 遺伝子発現 / 四肢 / 器官形成 / 形態形成
Outline of Final Research Achievements

Amphibians have high regenerative capacity. For example, they can regenerate a limb after amputation. However, difficulty in manipulating gene expression in spatially restricted manner has so far hindered elucidation of the molecular mechanisms of regeneration in amphibians. In this study, I applied two types of gene induction systems, IR-LEGO and temperature stimulator to amphibians and enabled local gene induction. As a result, function of a specific gene has been revealed by single cell level gene induction by IR-LEGO. Furthermore, gene induction exclusively in a specific region in a limb has been done by using temperature stimulator.

Report

(4 results)
  • 2016 Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Research-status Report
  • 2013 Research-status Report

Research Products

(28 results)

All 2016 2015 2014 2013 Other

All Journal Article Presentation Remarks

  • [Journal Article] Developmental stages for the divergence of relative limb length between a twig and a trunk-ground Anolis lizard species2015

    • Author(s)
      Wakasa, H., Cadiz, A., Echenique-Diaz, L.M. Iwasaki, W.M. Kamiyama, N., Nishimura, Y., Yokoyama, H., Tamura, K. and Kawata, M.
    • Journal Title

      Journal of Experimental Zoology (Part B: Mol. Dev. Evol.)

      Volume: 未定 Pages: 410-423

    • DOI
      10.1002/jez.b.22627
    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evidence for an amphibian sixth digit2015

    • Author(s)
      Hayashi S., Kobayashi T., Yano T., Kamiyama N., Egawa S., Seki R., Takizawa K., Okabe M., Yokoyama H. and Tamura K.
    • Journal Title

      Zoological Letters

      Volume: 1 Pages: 17-17

    • DOI
      10.1186/s40851-015-0019-y
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Epigenetic modification maintains intrinsic limb-cell identity in Xenopus limb bud regeneration2015

    • Author(s)
      Hayashi, S., Kawaguchi, A., Uchiyama, I., Kawasumi-Kita, A., Kobayashi, T., Nishide, H., Tsutsumi, R., Tsuru, K., Inoue, T., Ogino, H., Agata, K., Tamura, K. and *Yokoyama, H.
    • Journal Title

      Dev Biol.

      Volume: 406 Pages: 271-282

    • DOI
      10.1016/j.ydbio.2015.08.013
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] AP-2β is a transcriptional regulator for determination of digit length in tetrapods2015

    • Author(s)
      Seki R, Kitajima K, Matsubara H, Suzuki T, Saito D, Yokoyama H, Tamura K.
    • Journal Title

      Developmental Biology

      Volume: 407 Pages: 75-89

    • DOI
      10.1016/j.ydbio.2015.08.006
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Application of local gene induction by infrared laser-mediated microscope and temperature stimulator to amphibian regeneration study2015

    • Author(s)
      Kawasumi, A., Hayashi, T., Kobayashi, T., Nagayama, C., Hayashi, S., Kamei, Y., Morishita, Y., T Takeuchi, T., Tamura, K., Yokoyama, H.
    • Journal Title

      Development Growth and Differentiation

      Volume: 57 Pages: 601-613

    • DOI
      10.1111/dgd.12241
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Roles of Hippo signaling pathway in size control of organ regeneration2015

    • Author(s)
      Hayashi, S., Yokoyama, H. and Tamura, K.
    • Journal Title

      Development Growth and Differentiation

      Volume: 未定

    • DOI
      10.1111/dgd.12212
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Altered developmental events in the anterior region of the chick forelimb give rise to avian-specific digit loss.2014

    • Author(s)
      Naoki Nomura, Hitoshi Yokoyama and Koji Tamura
    • Journal Title

      Dev Dyn

      Volume: published online Pages: 741-52

    • DOI
      10.1002/dvdy.24117
    • Related Report
      2014 Research-status Report 2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Growth and differentiation of a long bone in limb development, repair and regeneration.2014

    • Author(s)
      S. Egawa, S. Miura, H. Yokoyama, T. Endo and K. Tamura
    • Journal Title

      Development, Growth and Differentiation

      Volume: 56 Pages: 410-24

    • DOI
      10.1111/dgd.12136
    • NAID
      40020109556
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transcriptional regulators in the Hippo signaling pathway control organ growth in Xenopus tadpole tail regeneration.2014

    • Author(s)
      S. Hayashi, H. Ochi, H. Ogino, A. Kawasumi, Y. Kamei, K. Tamura and H. Yokoyama
    • Journal Title

      Developmental Biology

      Volume: 396 Pages: 31-41

    • DOI
      10.1016/j.ydbio.2014.09.018
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] アフリカツメガエルから見た四肢再生実現へのステップ2014

    • Author(s)
      横山仁、林真一、川住愛子、砂川奈都召、田村宏治
    • Journal Title

      実験医学

      Volume: 32(1) Pages: 22-28

    • Related Report
      2013 Research-status Report
  • [Journal Article] Yap1, transcription regulator in the Hippo signaling pathway, is required for Xenopus limb bud regeneration2014

    • Author(s)
      Hayashi S, Tamura K, Yokoyama H
    • Journal Title

      Dev Biol

      Volume: 388 Pages: 57-67

    • DOI
      10.1016/j.ydbio.2014.01.018
    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Growth and differentiation of a long bone in limb development, repair and regeneration2014

    • Author(s)
      Egawa, S., Miura, S., Yokoyama, H., Endo, T., and Tamura, K.
    • Journal Title

      Development Growth and Differentiation

      Volume: 未定

    • NAID
      40020109556
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 両生類の皮膚再生と四肢再生の関係から見る,両生類再生研究の哺乳類への応用の可能性2013

    • Author(s)
      田村宏治、大塚理奈、川住愛子、横山仁
    • Journal Title

      瘢痕・ケロイド治療ジャーナル

      Volume: 7 Pages: 1-4

    • Related Report
      2013 Research-status Report
  • [Journal Article] 四肢再生における脱分化、再分化と細胞記憶2013

    • Author(s)
      林真一、矢野十織、川住愛子、田村宏治、横山仁
    • Journal Title

      実験医学

      Volume: 31(13) Pages: 2075-2082

    • Related Report
      2013 Research-status Report
  • [Presentation] 四肢と皮膚の完全再生を可能にする分子・細胞メカニズムの探求2016

    • Author(s)
      横山仁
    • Organizer
      日本解剖学会 シンポジウム再生研究の新機軸 ―不可能を可能とする内在性プログラムの探索―
    • Place of Presentation
      ビッグパレットふくしま(福島県郡山市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Skin regeneration of Xenopus, as a model for scar-free skin regeneration in adulthood2016

    • Author(s)
      Hitoshi Yokoyama, Rina Otsuka, Aiko Kawasumi-Kita, Tamae Maruoka and Koji Tamura
    • Organizer
      Aquatic model organisms for human disease and toxicology research
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ツメガエルにおける局所的な遺伝子発現の操作法の確立と再生研究への応用2015

    • Author(s)
      横山仁, 川住愛子, 林利憲, 小林託也, 永山誓史, 林真一, 亀井保博, 森下喜弘, 竹内隆, 田村宏治
    • Organizer
      日本ツメガエル研究集会
    • Place of Presentation
      秋田温泉さとみ(秋田市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] アフリカツメガエルを用いた皮膚再生の研究 ‐これまでとこれから‐2015

    • Author(s)
      横山仁
    • Organizer
      次世代両生類研究会
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Year and Date
      2015-08-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] アフリカツメガエルにおける皮膚の完全再生に寄与する細胞の起源2015

    • Author(s)
      大塚理奈, 有泉高史, 永山誓史, 川住愛子, 田村宏治, 横山仁
    • Organizer
      日本動物学会東北支部大会
    • Place of Presentation
      東北大学農学部(仙台市)
    • Year and Date
      2015-08-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] アフリカツメガエルにおける瘢痕を残さない皮膚再生 ‐再生に寄与する細胞はどこから来るのか?-2015

    • Author(s)
      横山仁、大塚理奈、有泉高史、永山誓史、川住愛子、田村宏治
    • Organizer
      日本再生医療学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Research-status Report
  • [Presentation] Cellular origin of blastema-like cells contributing to scarless skin regeneration of metamorphosed Xenopus laevis2014

    • Author(s)
      Rina Otsuka, Chikashi Nagayama, Aiko Kawasumi, Takashi Ariizumi , Koji Tamura, Hitoshi Yokoyama
    • Organizer
      日本発生生物学会
    • Place of Presentation
      ウインクあいち(名古屋市)
    • Year and Date
      2014-05-28 – 2014-05-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Cellular origin of blastema-like cells contributing to scarless2014

    • Author(s)
      Rina Otsuka, Chikashi Nagayama, Aiko Kawasumi, Takashi Ariizumi, Koji Tamura, Hitoshi Yokoyama
    • Organizer
      日本発生生物学会
    • Place of Presentation
      ウィンクあいち
    • Related Report
      2013 Research-status Report
  • [Presentation] Different cellular contribution of somite-derived tissues to limb regeneration in larval and adult Xenopus2013

    • Author(s)
      永山誓史、有泉高史 、田村宏治、横山仁
    • Organizer
      日本発生生物学会
    • Place of Presentation
      くにびきメッセ(島根県松江市)
    • Related Report
      2013 Research-status Report
  • [Presentation] 発生原理から見たアフリカツメガエルの器官再生2013

    • Author(s)
      横山仁
    • Organizer
      日本動物学会
    • Place of Presentation
      岡山大学
    • Related Report
      2013 Research-status Report
    • Invited
  • [Remarks] 弘前大学農学生命科学部教員紹介ページ

    • URL
      http://nature.cc.hirosaki-u.ac.jp/staff/hitoshi-yokoyama
    • Related Report
      2015 Annual Research Report
  • [Remarks] 弘前大学農学生命科学部教員紹介

    • URL
      http://nature.cc.hirosaki-u.ac.jp/public/hitoshi-yokoyama/
    • Related Report
      2014 Research-status Report
  • [Remarks] 田村研ホームページ 横山仁 研究業績リスト

    • URL
      http://www.biology.tohoku.ac.jp/lab-www/tamlab/members/yoko_pub.html
    • Related Report
      2013 Research-status Report
  • [Remarks] 東北大学 大学院生命科学研究科 教員紹介ページ 横山仁

    • URL
      http://www.lifesci.tohoku.ac.jp/teacher/t_yokoyamaj/
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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