The role of gammadelta T cells in the pathogenesis of interstitial pneumonia

• Project/Area Number: 25870092
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology; Respiratory organ internal medicine
• Research Institution: University of Tsukuba
• Principal Investigator: SEGAWA Seiji 筑波大学, 医学医療系, 助教 (60632239)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 全身性強皮症 / 間質性肺障害 / γδT細胞

Outline of Final Research Achievements

Interstitial pneumonia (IP) is a chronic progressive interstitial lung disease associated with high mortality and poor prognosis. However, the pathogenesis of IP remains to be elucidated. The goal of this study is to clarify the role of γδNKT (=CD161+ Vδ1+ γδT) cells in systemic sclerosis (SSc) patients with IP. The proportion of gammadelta NKT cells was significantly higher in SSc than healthy controls (HC), and correlated negatively with serum KL-6 levels in IP-positive SSc patients. γδNKT cells in IP-positive SSc patients showed higher production of CCL3 and lower production of IFN-γ than that in HC. Culture supernatant derived from IP-positive SSc patients promoted fibroblast proliferation, whereas that from HC did not. These finding suggest that γδNKT cells may play a regulatory role in the pathogenesis of IP in SSc patients, via IFN-γ. production.

Research Products

• [Journal Article] Involvement of CD161+Vδ1+γδT cells in systemic sclerosis: Association with interstitial pneumonia. (2014)
  • Author(s): Seiji Segawa, Daisuke Goto, Masanobu Horikoshi, Yuya Kondo, Naoto Umeda, Shinya Hagiwara, Masahiro Yokosawa, Tomoya Hirota, Haruka Miki, Hiroto Tsuboi, Hiroshi Ogishima, Takeshi Suzuki, Isao Matsumoto, Takayuki Sumida
  • Journal Title: Rheumatology Volume: 53 Pages: 2259-2269
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of CD161+ Vδ1+ γδT cells in systemic sclerosis: Association with interstitial pneumonia (2014)
  • Author(s): Seiji Segawa, Daisuke Goto, Masanobu Horikoshi, Yuya Kondo, Naoto Umeda, Shinya Hagiwara, Masahiro Yokosawa, Tomoya Hirota, Haruka Miki, Hiroto Tsuboi, Hiroshi Ogishima, Takeshi Suzuki, Isao Matsumoto, Takayuki Sumida.
  • Journal Title: Rheumatology Volume: -
  • Peer Reviewed
• [Presentation] The role of TCR Vδ1+ γδT cells in systemic sclerosis patients with interstitial pneumonitis (2014)
  • Author(s): Seiji Segawa, Daisuke Goto, Masanobu Horikoshi, Akira Iizuka, Isao Matsumoto, Takayuki Sumida
  • Organizer: 2014日本免疫学会総会・学術総会
  • Place of Presentation: 京都
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] 間質性肺炎合併強皮症患者におけるγδNKT細胞の機能解析 (2014)
  • Author(s): 瀬川誠司, 後藤大輔, 堀越正信, 飯塚晃、松本功, 住田孝之
  • Organizer: 第59回日本リウマチ学会総会・学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 間質性肺炎合併強皮症患者におけるTCR Vδ1+ NKT細胞の解析 (2013)
  • Author(s): 瀬川誠司, 後藤大輔, 堀越正信, 松本功, 住田孝之
  • Organizer: 第58回日本リウマチ学会総会・学術集会
  • Place of Presentation: 京都
• [Presentation] The regulatory role of TCR Vδ1+ γδT cells in systemic sclerosis patients with interstitial pneumonia (2013)
  • Author(s): Seiji Segawa, Daisuke Goto, Masanobu Horikoshi, Isao Matsumoto, Takayuki Sumida
  • Organizer: The 15th International Congress of Immunology
  • Place of Presentation: イタリア・ミラノ
• [Presentation] Involvement of TCR Vδ1+ NKT cells in systemic sclerosis: Association with interstitial pneumonia (2013)
  • Author(s): Seiji Segawa, Daisuke Goto, Masanobu Horikoshi, Yuya Kondo, Naoto Umeda, Shinya Hagiwara, Masahiro Yokosawa, Tomoya Hirota, Haruka Miki, Hiroto Tsuboi, Hiroshi Ogishima, Takeshi Suzuki, Isao Matsumoto, Takayuki Sumida.
  • Organizer: 2013 ACR/ARHP Annual Meeting
  • Place of Presentation: アメリカ合衆国・サンディエゴ
• [Presentation] Involvement of TCR Vδ1+ NKT cells in systemic sclerosis: Association with interstitial pneumonia (2013)
  • Author(s): Seiji Segawa, Daisuke Goto, Masanobu Horikoshi, Yuya Kondo, Naoto Umeda, Shinnya Hagiwara, Masahiro Yokosawa, Tomoya Hirota, Haruka Miki, Hiroto Tsuboi, Hiroshi Ogishima, Takeshi Suzuki, Isao Matsumoto and Takayuki Sumida
  • Organizer: 2013日本免疫学会総会・学術総会
  • Place of Presentation: 幕張