Functional analysis of anti-sense RNA and signal transduction in bacteria

• Project/Area Number: 25870115
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology); Applied microbiology
• Research Institution: Gunma University
• Principal Investigator: HIRAKAWA Hidetada 群馬大学, 先端科学研究指導者育成ユニット, 助教 (80431758)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: クオラムセンシング / 病原性 / アンチセンスＲＮＡ / オートインデューサー / 情報伝達 / ホモセリンラクトン / アンチセンスRNA / 感染症

Outline of Final Research Achievements

We found molecules that represses signal tranduction in bacteria. These are termed anti-sense RNA. We have the molecular mechanism of antisense RNA in photosynthetic bacterium and pathogens such as Escherichia coli and Pseudomonas aeruginosa. This studies aid us to create a new idea for anti-bacterial reagent that targets to signal transduction.

Research Products

• [Journal Article] Identification of a second two-component signal transduction system that controls fosfomycin tolerance and glycerol-3-phosphate uptake. (2015)
  • Author(s): 1.Kurabayashi K. Hirakawa Y, Tanimoto K, Tomita H, Hirakawa H
  • Journal Title: Journal of Bacteriology Volume: 197 Issue: 5 Pages: 861-871
  • DOI: 10.1128/jb.02491-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The role of the CpxAR two-component signal transduction system in control of fosfomycin resistance and carbon substrate uptake (2014)
  • Author(s): Kurabayashi K. Hirakawa Y, Tanimoto K, Tomita H, Hirakawa H
  • Journal Title: Journal of Bacteriology Volume: 196 Issue: 2 Pages: 248-256
  • DOI: 10.1128/jb.01151-13
  • Peer Reviewed / Open Access
• [Journal Article] 新しい細菌オートインデューサーによるクオラムセンシング機構 (2014)
  • Author(s): 平川秀忠、富田治芳
  • Journal Title: 感染・免疫・炎症 Volume: 44 Pages: 269-272
• [Journal Article] Interference of bacterial cell-to-cell communication: a new concept of antimicrobial chemotherapy breaks antibiotic resistance (2013)
  • Author(s): Hirakawa H, Tomita H
  • Journal Title: Frontiers in Microbiology Volume: 4-114 Pages: 1-14
  • DOI: 10.3389/fmicb.2013.00114
  • Peer Reviewed
• [Presentation] Trimethylamine-N-oxide感知応答Torシステムによる腸管出血性大腸菌O157のホスホマイシン耐性誘導 (2014)
  • Author(s): 倉林久美子、平川裕子、谷本弘一、富田治芳、平川秀忠
  • Organizer: 第６１回日本化学療法学会東日本支部総会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-29 – 2014-10-31
• [Presentation] 社会形成による大腸菌群の薬剤耐性と病原性制御 (2014)
  • Author(s): 平川秀忠
  • Organizer: 第６６回日本生物工学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2014-09-09 – 2014-09-11
  • Invited
• [Presentation] Reversible Control of Fosfomycin Resistance and Carbon Substrate Uptake by Two-component Signal Transduction System (2014)
  • Author(s): Kumiko Kurabayashi, Yuko Hirakawa, Koichi Tanimoto, Haruyoshi Tomita, Hidetada Hirakawa
  • Organizer: American Society for Microbiology 114th General Meeting
  • Place of Presentation: ボストン
  • Year and Date: 2014-05-17 – 2014-05-20
• [Presentation] アシルホモセリンラクトン新規サブタイプと細菌間コミュニケーション (2013)
  • Author(s): 平川秀忠
  • Organizer: 日本細菌学会 第７回若手研究者育成のためのワークショップ 若手研究者によるバイオフィルム研究ワークショップ
  • Place of Presentation: 国立感染症研究所
  • Invited
• [Book] 日本細菌学会誌 (2013)
  • Author(s): 平川秀忠、富田治芳
  • Total Pages: 11
  • Publisher: 日本細菌学会
• [Book] 実験医学 (2013)
  • Author(s): 平川秀忠:
  • Total Pages: 2
  • Publisher: 羊土社