Development of high-sensitive mitochondrial stress reporter for in vivo ROS-damage imaging

• Project/Area Number: 25870118
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science; Chemical biology
• Research Institution: Gunma University
• Principal Investigator: OIKAWA Daisuke 群馬大学, 生体調節研究所, 助教 (20455330)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 活性酸素 / 炎症シグナル / IL-1β / 酸化ストレスレポーター / in vivoイメージング

Outline of Final Research Achievements

ROS (reactive oxygen species) is a mitochondrial by-product, interfering with various kinds of biomolecule function. In this study, we developed novel reporter systems detecting mitochondrial stress status or inflammatory damage, and evaluated ROS-mediated damage at cellular level or in animal model.

Research Products

• [Journal Article] Positive contribution of IRE1α-XBP1 pathway to the expression of placental cathepsins. (2013)
  • Author(s): Oikawa D, Iwawaki T.
  • Journal Title: Biochem Biophys Res Commun. Volume: 433 Pages: 426-431
  • Peer Reviewed
• [Journal Article] The role of the unfolded protein response in diabetes mellitus. (2013)
  • Author(s): Iwawaki T, Oikawa D.
  • Journal Title: Semin Immunopathol. Volume: 35 Pages: 333-350
• [Journal Article] 生きているマウスで酸化ストレスを見えるように (2013)
  • Author(s): 及川大輔，岩脇隆夫
  • Journal Title: 化学と生物 Volume: 51 Pages: 361-363
• [Journal Article] Membrane lipid saturation activates IRE1α without inducing clustering. (2013)
  • Author(s): Kitai Y, Ariyama H, Kono N, Oikawa D, Iwawaki T, Arai H.
  • Journal Title: Genes Cells. Volume: 18 Pages: 798-809
  • Peer Reviewed
• [Presentation] Linear Ubiquitin binding of optineurin regulates NF-κB signaling. (2015)
  • Author(s): Nakazawa S, Ishii R, Oikawa D, Ishitani R, Nureki O, Tokunaga F.
  • Organizer: 2nd International symposium on "Protein modifications in Pathogenic Dysregulation of Signaling"
  • Place of Presentation: The University of Tokyo
  • Year and Date: 2015-01-23
• [Presentation] Optineurinの直鎖状ユビキチン結合を介したNF-κB 抑制と疾患との関連 (2014)
  • Author(s): 及川大輔、石井亮平、中澤世識、石谷隆一郎、濡木理、徳永文稔
  • Organizer: 第37回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Optineurin regulates NF-κB via linear ubiquitin binding. (2014)
  • Author(s): Nakazawa S, Oikawa D, Ishii R, Hatada I, Nureki O, Tokunaga F.
  • Organizer: International symposium on "Homeostasis through development, life, and diseases"
  • Place of Presentation: Gunma University
  • Year and Date: 2014-11-07
• [Presentation] OptineurinによるNF-κBシグナル制御の細胞機構と疾患 (2014)
  • Author(s): 及川大輔、石井亮平、中澤世識、石谷隆一郎、濡木理、徳永文稔
  • Organizer: 第9回臨床ストレス応答学会
  • Place of Presentation: 岡山大学
  • Year and Date: 2014-11-01 – 2014-11-02
• [Presentation] 新規LUBACシグナル複合体構成因子の探索と細胞機能解析 (2014)
  • Author(s): 及川大輔、中澤世識、徳永文稔
  • Organizer: 第66回日本細胞生物学会
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2014-06-11 – 2014-06-13
• [Presentation] High-sensitive in vivo Imaging of Oxidative Stress with OKD48 mice. (2013)
  • Author(s): Daisuke Oikawa, Ryoko Akai, Mio Tokuda, Takao Iwawaki.
  • Organizer: 日本組織培養学会 第86回大会
  • Place of Presentation: 独立行政法人産業技術総合研究所
  • Invited
• [Presentation] 胎盤での Cathepsin 遺伝子発現制御における IRE1alpha および XBP1 の役割 (2013)
  • Author(s): 岩脇隆夫、及川大輔
  • Organizer: 第60回北関東医学会総会
  • Place of Presentation: 群馬大学医学部刀城会館
• [Patent(Industrial Property Rights)] 炎症レポーターシステム (2014)
  • Inventor(s): 岩脇隆夫、及川大輔、石川智夫
  • Industrial Property Rights Holder: 岩脇隆夫、及川大輔、石川智夫
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-07-31
  • Overseas