| Project/Area Number |
25870119
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
Cell biology
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| Research Institution | Osaka University (2015)
Gunma University (2013-2014) |
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Principal Investigator |
SAKAGUCHI Aisa 大阪大学, 理学(系)研究科(研究院), 助教 (90608697)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 低分子量GTPase / 受精 / 線虫 / Rab11 / 膜輸送 / 初期胚 / オルガネラ / 表層顆粒 |
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| Outline of Final Research Achievements |
Fertilization triggers a dynamic conversion of intracellular components. We have found that small GTPase RAB-11 changes its localization and function dynamically during oocyte-to-embryo transition in C. elegans. In this study, we identified RAB-eleven-interacting protein-1 (REI-1) as a novel guanine nucleotide exchange factor (GEF) for RAB-11. REI-1 family proteins are conserved among metazoan, and its human homolog, SH3BP5 also exhibits a GEF activity toward Rab11. In C. elegans, REI-1 is expressed in the germline and co-localizes with RAB-11 on the Golgi membranes. The loss of REI-1 impaired the targeting of RAB-11 to the Golgi compartment and the cleavage furrow in early embryos, which resulted in cytokinesis delay. We suggest that REI-1 is a GEF specifically regulating the RAB-11 localization and functions in early embryos. More extensive studies of the REI-1 family proteins would uncover precise molecular mechanisms of the tissue-specific and spatiotemporal regulation for Rab11.
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