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Analysis of the molecular mechanism for small GTPase RAB-11

Research Project

Project/Area Number 25870119
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Developmental biology
Cell biology
Research InstitutionOsaka University (2015)
Gunma University (2013-2014)

Principal Investigator

SAKAGUCHI Aisa  大阪大学, 理学(系)研究科(研究院), 助教 (90608697)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords低分子量GTPase / 受精 / 線虫 / Rab11 / 膜輸送 / 初期胚 / オルガネラ / 表層顆粒
Outline of Final Research Achievements

Fertilization triggers a dynamic conversion of intracellular components. We have found that small GTPase RAB-11 changes its localization and function dynamically during oocyte-to-embryo transition in C. elegans. In this study, we identified RAB-eleven-interacting protein-1 (REI-1) as a novel guanine nucleotide exchange factor (GEF) for RAB-11. REI-1 family proteins are conserved among metazoan, and its human homolog, SH3BP5 also exhibits a GEF activity toward Rab11. In C. elegans, REI-1 is expressed in the germline and co-localizes with RAB-11 on the Golgi membranes. The loss of REI-1 impaired the targeting of RAB-11 to the Golgi compartment and the cleavage furrow in early embryos, which resulted in cytokinesis delay. We suggest that REI-1 is a GEF specifically regulating the RAB-11 localization and functions in early embryos. More extensive studies of the REI-1 family proteins would uncover precise molecular mechanisms of the tissue-specific and spatiotemporal regulation for Rab11.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (9 results)

All 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (4 results) Remarks (2 results)

  • [Journal Article] REI/SH3BP5 protein family: New GEFs for Rab11.2016

    • Author(s)
      Ken Sato, Aisa Sakaguchi, Miyuki Sato
    • Journal Title

      Cell Cycle

      Volume: 15 Issue: 6 Pages: 767-769

    • DOI

      10.1080/15384101.2015.1137710

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] REI-1 Is a Guanine Nucleotide Exchange Factor Regulating RAB-11 Localization and Function in C. elegans Embryos.2015

    • Author(s)
      Sakaguchi A, Sato M, Sato K, Gengyo-Ando K, Yorimitsu T, Nakai J, Hara T, Sato K, Sato K.
    • Journal Title

      Dev Cell

      Volume: 35(2) Issue: 2 Pages: 211-221

    • DOI

      10.1016/j.devcel.2015.09.013

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] REI-1は線虫の初期胚においてRAB-11の局在および機能を制御するGEFである2015

    • Author(s)
      坂口愛沙・佐藤美由紀・佐藤 健
    • Journal Title

      First Author's

      Volume: - Pages: 131

    • DOI

      10.7875/first.author.2015.131

    • Related Report
      2015 Annual Research Report
    • Open Access
  • [Presentation] 低分子量GTPase RAB-11の新規結合因子REI-1/REI-2は受精後のRAB-11再局在化を制御する2014

    • Author(s)
      坂口愛沙,佐藤美由紀,安藤恵子,佐藤克哉,中井淳一,佐藤健
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Novel binding partner of small GTPase RAB-11 regulates RAB-11 redistribution to Golgi after fertilization2014

    • Author(s)
      Aisa Sakaguchi, Miyuki Sato, Keiko Gengyo-Ando, Katsuya Sato, Junichi Nakai, Ken Sato
    • Organizer
      C. elegans Development, Cell Biology, Gene Expression Meeting in association with The 6th Asia-Pacific C. elegans Meeting
    • Place of Presentation
      奈良県奈良市
    • Year and Date
      2014-07-15 – 2014-07-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 線虫受精卵においてダイナミックに変化するRAB-11の動態制御機構の解析2014

    • Author(s)
      坂口愛沙,佐藤美由紀,安藤恵子,佐藤克哉,中井淳一,佐藤健
    • Organizer
      第66回日本細胞生物学会大会
    • Place of Presentation
      奈良県奈良市
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 受精における表層顆粒分泌の制御因子RAB-11に結合する新規因子の解析

    • Author(s)
      坂口愛沙,佐藤美由紀,安藤恵子,佐藤克哉,中井淳一,佐藤健
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場
    • Related Report
      2013 Research-status Report
  • [Remarks] 群馬大学生体調節研究所細胞構造分野ホームページ

    • URL

      http://traffic.dept.med.gunma-u.ac.jp/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 群馬大学生体調節研究所細胞構造分野

    • URL

      http://traffic.dept.med.gunma-u.ac.jp/index.html

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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