| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
After induction of hiPS cells to the hepatic lineage according to the published procedures, we enriched the cells expressing cell surface antigens expressed on liver progenitors by cell sorting using specific antibodies. Sorted cells exhibited a high proliferative potential. Those cells expressed immature hepatic genes, such as AFP and HNF4a. After expansion of those cells by serial passages, they were able to differentiate into either hepatocyte-like cells or cholangiocyte-like cells depending on the culture condition. These results suggest that self-renewable liver progenitors can be isolated from hiPS cells. They may be a useful source for producing a large amount of functional hepatocytes and cholangiocytes.
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