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Generation of hepatocyte from iPS cells and establishment of human liver chimeric mice

Research Project

Project/Area Number 25870145
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General anatomy (including histology/embryology)
General medical chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

KIDO Taketomo  東京大学, 分子細胞生物学研究所, 助教 (00401034)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsiPS細胞 / 肝臓 / 肝細胞 / 胆管上皮細胞
Outline of Final Research Achievements

After induction of hiPS cells to the hepatic lineage according to the published procedures, we enriched the cells expressing cell surface antigens expressed on liver progenitors by cell sorting using specific antibodies. Sorted cells exhibited a high proliferative potential. Those cells expressed immature hepatic genes, such as AFP and HNF4a. After expansion of those cells by serial passages, they were able to differentiate into either hepatocyte-like cells or cholangiocyte-like cells depending on the culture condition. These results suggest that self-renewable liver progenitors can be isolated from hiPS cells. They may be a useful source for producing a large amount of functional hepatocytes and cholangiocytes.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (7 results)

All 2015 2014 2013 Other

All Presentation (7 results)

  • [Presentation] ヒトiPS細胞由来肝前駆細胞の二分化能の解析2015

    • Author(s)
      木戸丈友、厚井悠太、田中稔、小林彩香、宮島篤
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトiPS細胞からの肝類洞内皮細胞への分化誘導と成熟肝細胞の作製への応用2015

    • Author(s)
      厚井悠太、木戸丈友、小林彩香、宮島篤
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトiPS細胞由来成熟肝細胞の誘導における肝非実質細胞の役割2015

    • Author(s)
      小林彩香、木戸丈友、 厚井悠太、 宮島篤
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトiPS細胞から肝類洞内皮細胞の分化誘導2014

    • Author(s)
      木戸 丈友、伊藤 利将、厚井 悠太、宮島 篤
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都、国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトiPS細胞からの機能的肝細胞の大量調製法の確立2014

    • Author(s)
      木戸 丈友、田中 稔、篠原 満利恵、Wenjin Xiao、酒井 康行、宮島 篤
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都、国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Generation of liver progenitors from human iPS cells2013

    • Author(s)
      Taketomo Kido, Minoru Tanaka, Yasuyuki Sakai, Atsushi Miyajima
    • Organizer
      第20回肝細胞研究会
    • Place of Presentation
      大阪、大阪国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] 創薬研究に向けたiPS細胞から機能的肝組織の構築へ

    • Author(s)
      木戸 丈友
    • Organizer
      バイオテクノロジー懇談会
    • Place of Presentation
      東京、東京大学
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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