| Project/Area Number |
25870146
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
Bio-related chemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology (2014-2015)
The University of Tokyo (2013) |
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Principal Investigator |
Kawakami Takashi 国立研究開発法人産業技術総合研究所, 創薬分子プロファイリング研究センター, 研究員 (60638881)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | PUREシステム / mRNAディスプレイ / Nメチルペプチド / Elongation factor P / 分子進化 / ペプトイド / Nアルキルペプチド / プロテオミクス / 抗体 / Nアルキルアミノ酸 / ペプチド / リボソーム / ケミカルバイオロジー / タンパク質 / tRNA / リガンド |
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| Outline of Final Research Achievements |
By the addition of elongation factor P (EF-P) to an E. coli reconstituted cell-free translation system, PURE system (Protein-synthesis Using Recombinant Elements systen), we successfully evaluated translation efficiency of cyclic N-alkyl amino acids in a library of mRNA-displayed highly N-alkylated polycyclic peptidomimetics.
By using bio-orthognal chemical reaction or enzymatic reaction, we developed a novel method to incorporate electorically-charged N-alkyl amino acids to ribosomally synthesized peptides.
By combining the PURE system with an mRNA display method, we successfully in vitro-evolved cyclized N-alkyl peptides targeted to VEGFR2 expressed in living human cells.
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