Evolutional study of the organellar genome replication enzyme by the genetic substitution in photosynthetic eukaryotes

• Project/Area Number: 25870155
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology; Evolutionary biology
• Research Institution: The University of Tokyo
• Principal Investigator: MORIYAMA TAKASHI 東京大学, 総合文化研究科, 助教 (80534346)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 紅藻 / 真核光合成生物 / オルガネラ / 葉緑体 / ミトコンドリア / ゲノム複製 / 細胞内局在 / 進化 / オルガネラDNA / ゲノム複製装置 / シアニジオシゾン

Outline of Final Research Achievements

We examined subcellular localization of enzymes related to replication of organellar genome in two red algae Cyanidioschyzon merolae and Porphyridium purpureum by observation of GFP-fusion protein, and indicated that the plastids and mitochondria contain markedly different components of replication machinery. These results and phylogenetic analyses showed that the enzymes related to organellar replication have been frequently replaced in the evolution of photosynthetic eukaryotes.

Research Products

• [Journal Article] Characterization of three putative xylulose-5-phosphate/fructose-6-phospate phosphoketolases in the cyanobacterium Anabaena sp. PCC 7120 (2015)
  • Author(s): Moriyama, T., Tajima, N., K., Sekine, K. and Sato, N
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Subcellular distribution of central carbohydrate metabolism pathways in the red alga Cyanidioschyzon merolae (2014)
  • Author(s): Moriyama, T., Sakurai, K., Sekine, K. and Sato, N
  • Journal Title: Planta Volume: 240 Issue: 3 Pages: 585-598
  • DOI: 10.1007/s00425-014-2108-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Enzymes involved in organellar DNA replication in photosynthetic eukaryotes (2014)
  • Author(s): Moriyama, T. and Sato, N
  • Journal Title: Frontiers in Plant Science Volume: 5 Pages: 480-480
  • DOI: 10.3389/fpls.2014.00480
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Localization and phylogenetic analysis of enzymes related to organellar genome replication in the unicellular rhodophyte Cyanidioschyzon merolae. (2014)
  • Author(s): T. Moriyama, N. Tajima, K. Sekine, N. Sato
  • Journal Title: Genome Biol. Evol. Volume: 6 Issue: 1 Pages: 228-237
  • DOI: 10.1093/gbe/evu009
  • Peer Reviewed
• [Journal Article] Detailed identification of fatty acid isomers sheds light on the probable precursors of triacylglycerol accumulation in photoautotrophically grown Chlamydomonas reinhardtii. (2014)
  • Author(s): K. Sakurai, T. Moriyama, N. Sato
  • Journal Title: Eukaryot. Cell Volume: 13 Issue: 2 Pages: 256-266
  • DOI: 10.1128/ec.00280-13
  • Peer Reviewed
• [Journal Article] Analysis of the complete plastid genome of the unicellular red alga Porphyridium purpureum (2014)
  • Author(s): Tajima N, Sato S,Maruyama F,Kurokawa K,Ohta H, Tabata S, Sekine K, Moriyama T, Sato N
  • Journal Title: J Plant Res Volume: 127 Issue: 3 Pages: 389-397
  • DOI: 10.1007/s10265-014-0627-1
  • NAID: 40020051727
  • Peer Reviewed
• [Journal Article] Klebsormidium flaccidum genome reveals primary factors for plant terrestrial adaptation (2014)
  • Author(s): Koichi Hori, Fumito Maruyama, Takatomo Fujisawa, Tomoaki Togashi, Nozomi Yamamoto, Mitsunori Seo, Shusei Sato, Takuji Yamada, Hiroshi Mori, Naoyuki Tajima, Takashi Moriyama et al.
  • Journal Title: Nature Communications Volume: 印刷中
• [Presentation] 紅藻シアニジオシゾンにおける生理活性に対する有機物の添加効果の解析 (2015)
  • Author(s): 森山崇、毛利奈津美、佐藤直樹
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 紅藻Cyanidioschyzon merolaeの脂質代謝関連酵素の網羅的解析 (2015)
  • Author(s): 毛利奈津美、森山崇、関根康介、豊島正和、佐藤直樹
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 緑藻Chlamydomonas applanata NIES-2202における窒素・リン欠乏条件下での脂質蓄積および脂肪酸組成の解析 (2015)
  • Author(s): 平嶋孝志、豊島正和、森山崇、佐藤直樹
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 紅藻Cyanidioschyzon merolaeの脂質代謝関連酵素の網羅的解析 (2014)
  • Author(s): 毛利奈津美、森山崇、関根康介、豊島正和、佐藤直樹
  • Organizer: 植物脂質シンポジウム
  • Place of Presentation: 静岡市
  • Year and Date: 2014-11-28 – 2014-11-29
• [Presentation] 藻類におけるリン脂質合成系の研究 (2014)
  • Author(s): 佐藤直樹、毛利奈津美、森山崇
  • Organizer: 日本植物学会
  • Place of Presentation: 川崎市
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] 紅藻Cyanidioschyzon merolaeの脂質代謝に関する研究 (2014)
  • Author(s): 毛利奈津美、森山崇、関根康介、佐藤直樹
  • Organizer: 日本植物学会
  • Place of Presentation: 川崎市
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] Anabaena sp. PCC7120におけるホスホケトラーゼの同定 (2014)
  • Author(s): 森山崇、佐藤直樹
  • Organizer: 日本植物学会
  • Place of Presentation: 川崎市
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] Divergent and parallel losses of phosphatidylcholine biosynthesis in algae (2014)
  • Author(s): Sato N, Mori N, Ogura S, Moriyama T
  • Organizer: International Symposium on Plant Lipids
  • Place of Presentation: カナダ、グエルフ
  • Year and Date: 2014-07-06 – 2014-07-11
• [Presentation] Analysis of subcellular localization of lipid metabolism enzymes in the red alga Cyanidioschyzon merolae (2014)
  • Author(s): Mori N, Moriyama T, Sekine K, Sato N
  • Organizer: International Symposium on Plant Lipids
  • Place of Presentation: カナダ、グエルフ
  • Year and Date: 2014-07-06 – 2014-07-11
• [Presentation] Chlamydomonas debaryanaにおける窒素飢餓条件での脂質の蓄積と脂肪酸組成の変化についての研究 (2014)
  • Author(s): 小倉翔平、毛利奈津美、森山崇、関根康介、佐藤直樹
  • Organizer: 日本光合成学会
  • Place of Presentation: 奈良市
  • Year and Date: 2014-05-30 – 2014-05-31
• [Presentation] 紅藻Cyanidioschyzon merolaeにおける脂質代謝についての研究 (2014)
  • Author(s): 毛利奈津美、森山崇、関根康介、佐藤直樹
  • Organizer: 日本光合成学会
  • Place of Presentation: 奈良市
  • Year and Date: 2014-05-30 – 2014-05-31
• [Presentation] 紅藻シアニジオシゾンにおける細胞内局在解析に基づいた炭素代謝経路図の作製 (2014)
  • Author(s): 森山崇、佐藤直樹
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山大学（富山県富山市）
• [Presentation] 単細胞性紅藻シアニディオシゾンにおけるオルガネラDNA複製関連酵素の局在および系統解析 (2013)
  • Author(s): 森山崇、田島直幸、関根康介、佐藤直樹
  • Organizer: 日本植物学会
  • Place of Presentation: 北海道大学（北海道札幌市）
• [Remarks] 佐藤直樹研究室
  • URL: http://nsato4.c.u-tokyo.ac.jp/