Basic studies for the development of bone and periodontal regeneration system using small molecule compounds

• Project/Area Number: 25870188
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry; Bioorganic chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: YONEZAWA Takayuki 東京大学, 工学(系)研究科(研究院), 特任助教 (20410665)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 歯周病 / 骨芽細胞 / 低分子化合物 / 骨形成

Outline of Final Research Achievements

To develop strategies for bone-periodontal tissue regeneration using small-molecule compounds, the mode of action of harmine, which is an activator compound of osteoblast differentiation, was investigated. As a result, we found that BMP, Wnt and Hedgehog pathways are involved in the action of harmine on osteoblast differentiation.

Research Products

• [Journal Article] Nobiletin, a polymethoxy flavonoid, reduced endothelin-1 plus SCF-induced pigmentation in human melanocytes. (2015)
  • Author(s): Kim HJ, Yonezawa T, Teruya T, Woo JT, Cha BY.
  • Journal Title: Photochem Photobiol. Volume: 91 Issue: 2 Pages: 379-386
  • DOI: 10.1111/php.12400
  • Peer Reviewed
• [Journal Article] Antidiabetic effect of nepodin, a component of Rumex roots, and its modes of action in vitro and in vivo (2014)
  • Author(s): Ha BG, Yonezawa T, Son MJ, Woo JT, Ohba S, Chung UI, Yagasaki K.
  • Journal Title: Biofactors. Volume: 40 Issue: 4 Pages: 436-447
  • DOI: 10.1002/biof.1165
  • Peer Reviewed
• [Journal Article] Rosmarinic acid exerts an anti-osteoporotic effect in the RANKL-induced mouse model of bone loss by promotion of osteoblastic differentiation and inhibition of osteoclastic differentiation. (2014)
  • Author(s): Lee JW, Asai M, Jeon SK, Iimura T, Yonezawa T, Cha BY, Woo JT, Yamaguchi A
  • Journal Title: Mol Nutr Food Res Volume: 59 Issue: 3 Pages: 386-400
  • DOI: 10.1002/mnfr.201400164
  • Peer Reviewed / Open Access
• [Journal Article] Daidzein promotes glucose uptake through glucose transporter 4 translocation to plasma membrane in L6 myocytes and improves glucose homeostasis in type 2 diabetic model mice. (2014)
  • Author(s): Cheong SH, Furuhashi K, Ito K, Nagaoka M, Yonezawa T, Miura Y, and Yagasaki K.
  • Journal Title: Journal of Nutritional Biochemistry Volume: 25 Issue: 2 Pages: 136-143
  • DOI: 10.1016/j.jnutbio.2013.09.012
  • Peer Reviewed
• [Journal Article] Sodium dodecyl sulfate and sodium dodecyl benzenesulfonate are ligands for peroxisome proliferator-activated receptor γ (2013)
  • Author(s): Iida K, Yonezawa T, Choi SS, Nagai K, Woo JT.
  • Journal Title: The Journal of Toxicological Sciences Volume: 38 Issue: 5 Pages: 697-702
  • DOI: 10.2131/jts.38.697
  • NAID: 10031191728
  • ISSN: 0388-1350, 1880-3989
  • Peer Reviewed
• [Journal Article] Antihyperglycemic effect of equol, a daidzein derivative, in cultured L6 myocytes and ob/ob mice. (2013)
  • Author(s): Cheong SH, Furuhashi K, Ito K, Nagaoka M, Yonezawa T, Miura Y, and Yagasaki K.
  • Journal Title: Molecular Nutrition and Food Research Volume: 58 Issue: 2 Pages: 267-277
  • DOI: 10.1002/mnfr.201300272
  • Peer Reviewed
• [Presentation] カルノシン酸およびカルノソールの破骨細胞分化抑制作用 (2015)
  • Author(s): 米澤貴之、夏目矩行、禹 済泰、鄭 雄一
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸学院大学他、兵庫県
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] 恒常的 TSLP 高発現産生細胞を用いた TSLP 産生阻害化合物の探索 (2014)
  • Author(s): 白木 美香、瀬川 良佑、山下 紗緒里、米澤 貴之、車 炳允、禹 済泰、平澤 典保
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本、ホテル日航熊本他
• [Presentation] 破骨細胞の機能に対する Biselyngbyaside の作用 (2013)
  • Author(s): 照屋俊明、米澤貴之、間瀬直美、佐々木宏明、末永聖武、車炳允、禹済泰、永井和夫
  • Organizer: 第15回マリンバイオテクノロジー学会大会
  • Place of Presentation: 沖縄、沖縄県市町村自治会館
• [Presentation] 破骨細胞分化に対するカルノシン酸およびカルノソールの作用 (2013)
  • Author(s): 米澤貴之、夏目矩行、禹 済泰、鄭 雄一
  • Organizer: 第26回 日本動物細胞工学会2013年度大会
  • Place of Presentation: 福井、ホテルフジタ福井
• [Presentation] エクオールの抗糖尿病作用
  • Author(s): 丁善姫、古橋慶亮、伊東克己、長岡真聡、米澤貴之、三浦、矢ヶ崎一三
  • Organizer: 第７回日本ポリフェノール学会年次大会
  • Place of Presentation: 東京、東京農工大学