Elucidation of "rock-paper-scissors" mechanism of ABC transporters by molecular simulations
| Project/Area Number |
25870220
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Life / Health / Medical informatics
Biophysics
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
FURUTA TADAOMI 東京工業大学, 大学院生命理工学研究科, 助教 (10431834)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ABCトランスポーター / ATP / 基質 / 輸送 / カップリングヘリックス / 分子シミュレーション / 構造変化 / 構造機能相関 / 生物物理 |
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| Outline of Final Research Achievements |
ATP-binding cassette (ABC) transporters constitute a large superfamily of integral membrane proteins that transport several substrates through cellular membranes using the energy of binding, hydrolysis, and release of ATP. In this study, we investigated the roles of coupling helices (CHs) and the effect of ATP for bacterial ABC transporters MsbA and TM287/288 as targets by molecular simulations. Compering the wild type and CH mutants, we revealed the roles of each CH on conformational changes and the effect of the binding of ATP. These findings provide important insight into the structure-function relationship of ABC transporters.
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Report
(3 results)
Research Products
(10 results)
