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Functional analysis of novel GAP-43 phosphorylation sites in neuronal growth

Research Project

Project/Area Number 25870251
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cell biology
Neurochemistry/Neuropharmacology
Research InstitutionNiigata University

Principal Investigator

KAWASAKI Asami  新潟大学, 研究推進機構・超域学術院, 助教 (10609358)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords成長円錐 / JNK / GAP-43 / growth cone / 発生 / 再生
Outline of Final Research Achievements

GAP-43 is thought to be involved in the mechanisms regulating the growth of neuronal processes during development and axon regeneration. However, its role for the molecular signaling is poorly understood. Recently, we performed a quantitative phosphoproteomic analysis of axonal growth cones and identified the novel phosphorylation sites of GAP-43 (Ser96 and Thr172), which are extensively highly phosphorylated in in vivo. Using specific antibodies of phospho-GAP-43 at these sites, we identified JNK was a major kinase responsible for these sites. In GAP-43 S96A knock-in mice, axonal growth of the primary cultured neurons was reduced by 50 %, compared with that of wild-type mice. These results suggest that JNK-dependent phosphorylation of GAP-43 is one of the important signaling involved in axonal generation and regeneration.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (9 results)

All 2015 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results)

  • [Journal Article] Involvement of IQGAP family proteins in the regulation of mammalian cell cytokinesis2014

    • Author(s)
      Makoto Adachi, Asami Kawasaki, Hisashi Nojima, Eisuke Nishida and Sachiko Tsukita
    • Journal Title

      Genes to Cells

      Volume: 19 Issue: 11 Pages: 803

    • DOI

      10.1111/gtc.12179

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] In vivo phosphorylation sites of GAP-43, involved axonal growth, is phosphorylated by JNK12015

    • Author(s)
      小林大記、河嵜麻実、武内恒成、五十嵐道弘
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸国際会議場、神戸国際展示場
    • Year and Date
      2015-07-28 – 2015-07-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] JNK-mediated phosphorylation of GAP-43 promotes axonal growth2015

    • Author(s)
      河嵜麻実、小林大記、武内恒成、五十嵐道弘
    • Organizer
      第67回日本細胞生物学会大会
    • Place of Presentation
      タワーホール船堀(東京)
    • Year and Date
      2015-06-30 – 2015-07-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] JNK-mediated phosphorylation of GAP-43 promotes axonal growth2015

    • Author(s)
      河嵜 麻実、岡田 正康、武内 恒成、 崎村建司, 五十嵐 道弘
    • Organizer
      第5回新潟大学脳研究所共同研究拠点国際シンポジウム
    • Place of Presentation
      新潟大学脳研究所
    • Year and Date
      2015-03-05 – 2015-03-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] The novel JNK-dependent phosphorylation sites of GAP-43 promote axonal growt h2014

    • Author(s)
      河嵜 麻実、吉岡望、岡田 正康、武内 恒成、 五十嵐 道弘
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] GAP-43の新規リン酸化部位の解析2014

    • Author(s)
      河嵜麻実、岡田正康、吉岡望、野住素広、武内恒成、五十嵐道弘
    • Organizer
      第19回グリアクラブ
    • Place of Presentation
      KKR湯沢 ゆきぐに(新潟)
    • Related Report
      2013 Research-status Report
  • [Presentation] 成長円錐における網羅的なリン酸化タンパク質の探索2013

    • Author(s)
      河嵜麻実、武内恒成、野住素広、五十嵐道弘
    • Organizer
      Neuro2013
    • Place of Presentation
      京都国際会議場(京都)
    • Related Report
      2013 Research-status Report
  • [Presentation] 神経成長円錐マーカー分子GAP43新規リン酸化ドメインとその認識抗体の応用2013

    • Author(s)
      河嵜麻実、野住素広、武内恒成、五十嵐道弘
    • Organizer
      第35回神経組織培養研究会
    • Place of Presentation
      ホテル阪急エキスポパーク(大阪)
    • Related Report
      2013 Research-status Report
  • [Presentation] GAP-43の新規のリン酸化はJNKの下流で軸索伸長を促進する/The novel JNK-dependent phosphorylation sites of GAP-43 promote axonal growth2013

    • Author(s)
      河嵜麻実、岡田正康、吉岡望、野住素広、武内恒成、五十嵐道弘
    • Organizer
      第36回分子生物学会
    • Place of Presentation
      神戸ポートアイランド(神戸)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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