Epileptigenecity in Hippocampal screlosis

• Project/Area Number: 25870252
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology; Neurosurgery
• Research Institution: Niigata University
• Principal Investigator: KITAURA Hiroki 新潟大学, 脳研究所, 助教 (80401769)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: てんかん / イメージング / ヒト脳スライス標本 / 病態生理学 / 海馬硬化症 / 電気生理学 / 光学的イメージング / 海馬 / 神経回路

Outline of Final Research Achievements

Mesial temporal lobe epilepsy (MTLE) is the most frequent focal epileptic syndrome in adults, and the majority of complex partial seizures originate from the mesial structures of temporal lobe, including the hippocampus. Due to the sufficient localization of the seizure onset and excellent results of resection, surgery has been considered as a standard care for these patients. However, the close mechanisms of epileptogenesis in these hippocampus have not been clarified. To investigate this issue, we performed flavoprotein fluorescence imaging and field potential recordings in vitro using samples of slice preparations of hippocampus surgically taken from patients with MTLE. Hippocampal slices were prepared from surgically resected brain tissue taken from seven intractable epileptic patients. We found that electrically evoked activity was enhanced in the subiculum in all of the MTLE group. Further, we noticed that mossy fiber sprouting may occured in the severely sclerotic hippocampus.

Research Products

• [Journal Article] The kick-in system: a novel rapid knock-in strategy. (2014)
  • Author(s): Tomonoh, Y., Deshimaru, M., Araki, K., Miyazaki, Y., Arasaki, T., Tanaka, Y., Kitamura, H., Mori, F., Wakabayashi, K., Yamashita, S., Saito, R., Itoh, M., Uchida, T., Yamada, J., Migita, K., Ueno, S., Kitaura, H., Kakita, A., Lossin, C., Takano, Y., Hirose, S.
  • Journal Title: PLoS One. Volume: 9 Issue: 2 Pages: 88549-88549
  • DOI: 10.1371/journal.pone.0088549
  • Peer Reviewed / Open Access
• [Journal Article] Ligand-based molecular MRI;0-17 JJVCPE amyloid imaging in transgenic mice (2014)
  • Author(s): Suzuki K, Igarashi H, Huber VJ, Kitaura H, Kwee IL, Nakada T
  • Journal Title: J Neuroimaging Volume: (受理済, 電子出版済) Issue: 6 Pages: 595-598
  • DOI: 10.1111/jon.12091
  • Peer Reviewed / Open Access
• [Journal Article] Optical imaging of human epileptogenic tissues in vitro. (2013)
  • Author(s): Kitaura H, Kakita A
  • Journal Title: Neuropathology Volume: 33 Issue: 4 Pages: 469-474
  • DOI: 10.1111/neup.12017
  • Peer Reviewed
• [Journal Article] Significance of horizontal propagation of synchronized activities in human epileptic neocortex investigated by optical imaging and immunohistological study. (2013)
  • Author(s): Hiraishi T, Kitaura H, Oishi M, Fukuda M, Kameyama S, Takahashi H, Kakita A, Fujii Y.
  • Journal Title: Epilepsy Res. Volume: 104 Issue: 1-2 Pages: 59-67
  • DOI: 10.1016/j.eplepsyres.2012.09.014
  • Peer Reviewed
• [Presentation] Epileptogenic mechanisms in mesial temporal lobe epilepsy: an in vitro optical imaging study of resected human hippocampus specimens (2014)
  • Author(s): Kitaura H
  • Organizer: Society for Neuroscience
  • Place of Presentation: Walter E. Washington Convention Center (Washington DC, USA)
  • Year and Date: 2014-11-14
• [Presentation] 視床下部過誤腫におけるてんかん原性のin vitro脳スライス標本を用いた検討 (2014)
  • Author(s): 北浦弘樹
  • Organizer: 日本神経病理学会
  • Place of Presentation: 学術総合センター（東京都千代田区）
  • Year and Date: 2014-06-06
• [Presentation] 海馬近傍に生じたPeriventriuclar nodular heterotopiaの1例：光学的イメージング法による神経ネットワークの解析 (2013)
  • Author(s): 北浦弘樹、大石誠、武井延之、付永娟、平石哲也、福多真史、高橋均、澁木克栄、藤井幸彦、柿田明美
  • Organizer: 日本神経病理学会
  • Place of Presentation: 東京
• [Presentation] Characteristic neuronal activities of patients with mesial temporal lobe epilepsy: an in vitro imaging study of the resected non-sclerotic hippocampus (2013)
  • Author(s): Hiroki Kitaura, Hiroshi Masuda, Hiroshi Shimizu, Hiroshi Shirozu, Hiroatsu Murakami, Hitoshi Takahashi, Shigeki Kameyama, Akiyoshi Kakita
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego, USA
• [Book] Epilepsy (2014)
  • Author(s): 北浦弘樹、柿田明美
  • Total Pages: 141
  • Publisher: メディカルレビュー