Bioluminescence imaging for monitoring the expression of BDNF gene; Toward drug development for neural and psychiatric disorders
| Project/Area Number |
25870256
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pharmacology in pharmacy
Neurophysiology / General neuroscience
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| Research Institution | University of Toyama |
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Principal Investigator |
Fukuchi Mamoru 富山大学, 大学院医学薬学研究部(薬学), 助教 (40432108)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | BDNF / ルシフェラーゼ / イメージング / 発光イメージング / 遺伝子発現 |
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| Outline of Final Research Achievements |
Brain-derived neurotrophic factor (BDNF) plays an important role in expressing long-lasting changes in neural functions such as learning and memory, and is associated with neural and psychiatric disorders. We recently generated a novel transgenic mouse strain, termed BDNF-Luc mouse, to visualize change in BDNF expression in living cells and in mice using bioluminescence imaging. In this study, I successfully detected bioluminescence signal and monitored time-dependent changes in the expression of BDNF gene in vitro and in vivo. Furthermore, I constructed a novel screening system to identify compounds and drugs that induce the expression of BDNF gene in neurons. Because BDNF inducers are candidate drugs for psychiatric disorders like depression, these results obtained by this study would contribute to drug development for neural and psychiatric disorders in future.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Neuromodulatory effect of Gαs- or Gαq-coupled GPCR on NMDAR selectively activates the NMDAR/Ca2+/calcineurin/CREB-regulated transcriptional coactivator 1 (CRTC1) pathway to effectively induce Bdnf expression in neurons.2015
Author(s)
Fukuchi M, Tabuchi A, Kuwana Y, Watanabe S, Inoue M, Takasaki I, Izumi H, Tanaka A, Inoue R, Mori H, Komatsu H, Takamori H, Okuno H, Bito H, Tsuda M.
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Journal Title
J Neurosci.
Volume: 35
Issue: 14
Pages: 5606-5624
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Type II pyrethroid deltamethrin produces antidepressant-like effects in mice.2013
Author(s)
Takasaki I, Oose K, Otaki Y, Ihara D, Fukuchi M, Tabuchi A, Tsuneki H, Tabuchi Y, Kondo T, Saitoh A, Yamada M, Tsuda M.
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Journal Title
Behav Brain Res.
Volume: 257
Pages: 182-188
DOI
Related Report
Peer Reviewed
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