Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Outline of Final Research Achievements |
The aim of this study is to purify and identify the mycorrhiza facilitation substances produced by mycorrhiza helper bacteria. Newly artificial medium was developed to purify its substance. Helper bacteria showed the enhancement of hyphal growth when valine put into the media as nitrogen source. In addition, crude substances were extracted from spent broth of helper bacteria using ethyl acetate, which showed effect of hyphal enhancement. On the other hand, newly bacteria having the hyphal enhancement activity on Fusarium oxysporum were isolated from rhizosphere soil. The sequencing analysis showed that the bacteria were Arthrobacter spp.
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