| Project/Area Number |
25870291
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry
Bio-related chemistry
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| Research Institution | Gifu University |
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Principal Investigator |
Ohno Satoshi 岐阜大学, 工学部, 助教 (10345796)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | タンパク質 / 非標準アミノ酸 / ナノカプセル / アミノアシルtRNA合成酵素 / カプセル |
|---|
| Outline of Final Research Achievements |
Aquifex aeolicus lumazine synthase (AaLS) forms hollow-icosahedral capsule by self-assembling of 60 subunits. A negatively charged lumazine synthase mutant( AaLS_Neg ) is expected as a bio-nanocapsule which can be used for a DDS. In this study, we prepared several AaLS_Neg mutants, each carrying an azido-tyrosine at predetermined positions 22, 40, 44, 57, 91, respectively. The synthesized AaLS_Neg mutant containing azido-tyrosine at position 22 was covalently crosslinked via azide group by UV irradiation. And then, this photo-crosslinked product was separated on a gel filtration column. As a result, we observed that the encapsulation efficiency of photo-crosslinked product was increased.
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