Physiological survival mechanism of bacterial community inhabiting in oligotrophic environments
| Project/Area Number |
25870295
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental dynamic analysis
Ecology/Environment
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| Research Institution | Shizuoka University |
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Principal Investigator |
KIMURA Hiroyuki 静岡大学, 理学(系)研究科(研究院), 准教授 (30377717)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | バクテリア / 海洋細菌 / 極限環境 / 貧栄養環境 / トランスクリプトーム / RNA / 生存戦略 / RNA発現 / small RNA / 定量RT-PCR |
|---|
| Outline of Final Research Achievements |
Transfer-messenger RNA is small RNA that employs both tRNA and mRNA-like properties as it rescues stalled ribosomes during nutrient shortage. Here, we surveyed transcriptional responses of transfer-messenger RNA-containing bacterial strains in the medium of various nutrient conditions. Consequently, transfer-messenger RNA showed significant up-regulation in the nutrient-limiting growth conditions.
We collected seawater samples from open ocean (oligotrophic environment) and coastal area (eutrophic environment). We then performed transcriptomic analyses targeting total RNA extracted from the bacterial communities in the seawater samples. Transfer-messenger RNA was expressed at significantly higher levels in the seawater sample derived from the open ocean. In total, our results suggest the importance of transfer-messenger RNA as essential components for nutrient-limiting growth conditions in these bacteria.
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Quantitative analysis of coenzyme F430 in environmental samples: a new diagnostic tool for methanogenesis and anaerobic methane oxidation2014
Author(s)
Kaneko, M., Takano, Y., Chikaraishi, Y., Ogawa, N.O., Asakawa, S., Watanabe, K., Shima, S., Krueger, M., Matsushita, M., Kimura, H., Ohkouchi, N.
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Journal Title
Analytical Chemistry
Volume: 86
Issue: 7
Pages: 3633-3638
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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