Development of a chemical sensor chip based on Xenopus laevis oocytes expressing chemical receptors
| Project/Area Number |
25870323
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
Device related chemistry
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| Research Institution | Kanagawa Academy of Science and Technology (2015)
Toyohashi University of Technology (2013-2014) |
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Principal Investigator |
Misawa Nobuo 公益財団法人神奈川科学技術アカデミー, 人工細胞膜システムグループ, 研究員 (70442530)
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| Research Collaborator |
MITSUNO Hidefumi
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 化学センサ / アフリカツメガエル卵母細胞 / 化学受容体 / 二電極膜電位固定法 / 細胞アレイ |
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| Outline of Final Research Achievements |
With the aim of developing a microfabricated chemical sensor chip combined with African clawed frog (Xenopus laevis) oocytes expressing chemical receptors by genetic engineering, I made a compact fluidic device integrated with microelectrodes that could measure the cells’ responses to chemical stimuli. Plural Xenopus oocytes could be trapped one-by-one in the fluidic device along with the flow at the same time as the electrodes’ automatic connection to each oocyte. The cell-based chemical sensor chip successfully detected a target chemical substance. Furthermore, for odorant detection using Xenopus oocytes expressing olfactory receptors, I additionally attempted to construct an odorant solubilization system based on a microfluidic device for gas-liquid mixing. The fluidic channel was filled with porous material such as filter papers. In this project, I succeeded in development of a Xenopus oocyte array fluidic device with microelectrodes for electrophysiological measurement.
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Report
(4 results)
Research Products
(17 results)
