Imaging of Tissue Regeneration and Tissue Engineering using Bioavailable molecular probes

• Project/Area Number: 25870359
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering; Nanobioscience
• Research Institution: Kyoto University
• Principal Investigator: Kimura Yu 京都大学, 健康長寿社会の総合医療開発ユニット, 特定准教授 (90566027)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 分子プローブ / デンドリマー / 磁気共鳴イメージング / MRI / ガドリニウム / 分子イメージング

Outline of Final Research Achievements

Novel Gadolinium (Gd)-Magnetic Resonance Imaging (MRI) probes with high-sensitivity were succeeded to synthesize and introduced with functional groups for conjugation of target ligands to specific cells. Complex molecules of novel Gd-MRI contrast agent and antibody fragment or polyethyleneglycol (PEG) was then obtained after the reaction of reduced antibody fragment and activated-PEG. The affinity of cell-specific labeling was evaluated with quartz crystal microbalance measurement and the no change of antigen-binding ability was confirmed before and after the conjugation of Gd-MRI contrast agent. Through evaluation of binding affinity to cultured cells and of body distribution after in vivo administration, many findings to clarify the cell distribution in tissue regeneration process were obtained.

Research Products

• [Journal Article] Synthesis and functional evaluation of chiral dendrimer-triaminecoordinated Gd complexes with polyaminoalcohol end groups as highly sensitive MRI contrast agents (2015)
  • Author(s): Miyake Y, Kimura Y, Orito N, Imai H, Matsuda T, Toshimitsu A, Kondo T.
  • Journal Title: Tetrahedron Volume: 71 Issue: 26-27 Pages: 4438-4444
  • DOI: 10.1016/j.tet.2015.04.050
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Pharmacokinetics of chiral dendrimer-triamine-coordinated Gd-MRI contrast agents evaluated by in vivo MRI and estimated by in vitro QCM (2015)
  • Author(s): Miyake Y, Ishikawa S, Kimura Y, Son A, Imai H, Matsuda T, Yamada H, Toshimitsu A, Kondo T.
  • Journal Title: Sensors Volume: 15 Issue: 12 Pages: 31973-31986
  • DOI: 10.3390/s151229900
  • NAID: 120007141563
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] HER2 部分抗体を結合した新規キラルデンドリマートリアミン配位 Gd-MRI 造影剤の合成と機能評価 (2014)
  • Author(s): 木村祐, 沖塚真珠美, 三宅由花, 松田哲也, 今井宏彦, 年光昭夫, 近藤輝幸
  • Organizer: 日本化学会第94春季年会
  • Place of Presentation: 名古屋大学東山キャンパス（名古屋市）
• [Book] Magnetic nanoparticles for multimodal bio-imaging (In Magnetic Nanoparticles: Synthesis, Physicochemical Properties and Role in Biomedicine; Sabbas, Nora P., Eds.) (2014)
  • Author(s): Kondo, Teruyuki; Kimura, Yu; Yamada, Hisatsugu; Toshimitsu, Akio
  • Total Pages: 24
  • Publisher: Nova Science Publishers
• [Book] 「生体高分子（タンパク質）」（田畑 泰彦編; 細胞の3次元組織化―その最先端技術と材料技術） (2014)
  • Author(s): 木村 祐
  • Total Pages: 5
  • Publisher: メディカルドゥ
• [Remarks] 京都大学学際融合教育研究推進センター先端医工学研究ユニット 近藤研究室ホームページ
  • URL: http://www.probe.abe.kyoto-u.ac.jp