NMR analysis of the conformationl change of an Arf-family Small G Protein

• Project/Area Number: 25870372
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry; Biophysics
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Okamura Hideyasu 国立研究開発法人理化学研究所, 生命システム研究センター, 研究員 (50620928)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ダイナミクス / 新規拡張model-free解析法 / 無細胞蛋白質合成システム

Outline of Final Research Achievements

The purpose of this study is to elucidate the molecular mechanism of the conformational change of an Arf-family Small G protein by using NMR relaxation analysis. Nicotiana tabacum and Human Arl8s were produced by a cell-free system that is capable of Advanced isotope-labeling techniques. The relaxation data of Arl8s were obtained and analyzed. In addition, I developed an extended model-free analysis that is applicable to macromolecular high concentrated systems like biological cells.

Research Products

• [Journal Article] Accurate and molecular-size-tolerant NMR quantitation of diverse components in solution (2016)
  • Author(s): Okamura, H., Nishimura, H., Nagata, T., Kigawa, T., Watanabe, T. and Katahira M.
  • Journal Title: Sci. Rep. Volume: 6(21742) Issue: 1 Pages: 1-12
  • DOI: 10.1038/srep21742
  • NAID: 120005705947
  • Peer Reviewed / Open Access
• [Journal Article] NMRで分子混雑環境におけるタンパク質の挙動を調べる (2014)
  • Author(s): 岡村英保、木川隆則
  • Journal Title: 細胞工学 Volume: 33 Pages: 830-834
  • Peer Reviewed
• [Journal Article] Conformational change of Sos-derived proline-rich peptide upon binding Grb2 N-terminal SH3 domain probed by NMR (2013)
  • Author(s): Kenji Ogura & Hideyasu Okamura
  • Journal Title: Scientific Reports Volume: 3 Issue: 1 Pages: 2913-2913
  • DOI: 10.1038/srep02913
  • NAID: 120005348191
  • Peer Reviewed
• [Presentation] 新規拡張model-free解析法により明らかになった分子混雑環境下での蛋白質運動 (2015)
  • Author(s): 岡村英保, 栃尾尚哉, 杉山修世, 伊東優拡, 渡部暁, 優乙石, フェイグマイケル, 杉田有冶, 木川隆則
  • Organizer: 第54回NMR討論会
  • Place of Presentation: 津田沼，千葉
  • Year and Date: 2015-11-06
• [Presentation] Analysis of Protein Dynamics in Macromolecular Crowding Environment (2015)
  • Author(s): Hideyasu Okamura, Takanori Kigawa
  • Organizer: 56th Experimental Nuclear Magnetic Resonance Conference
  • Place of Presentation: Asilomar Conference Center (USA)
  • Year and Date: 2015-04-19 – 2015-04-24
• [Presentation] Analysis of Protein Dynamics in Macromolecular Crowding Environment (2015)
  • Author(s): Hideyasu Okamura, Takanori Kigawa
  • Organizer: 56th Experimental Nuclear Magnetic Resonance Conference
  • Place of Presentation: Pacific Grove, USA
  • Year and Date: 2015-04-19
  • Int'l Joint Research
• [Presentation] 分子混雑環境に適用可能な新規拡張model-free解析法の開発 (2014)
  • Author(s): 岡村英保，栃尾尚哉，杉山修世，渡部暁，優乙石，フェイグマイケル，杉田有治，木川隆則
  • Organizer: 第５３回NMR討論会
  • Place of Presentation: 大阪大学（大阪府）
  • Year and Date: 2014-11-04 – 2014-11-06
• [Presentation] 分子量（横緩和時間）の差に由来する物質定量のゆがみを克服する新しい測定法の開発 (2014)
  • Author(s): 岡村英保，西村裕志，永田崇，木川隆則，渡辺隆司，片平正人
  • Organizer: 第５３回NMR討論会
  • Place of Presentation: 大阪大学（大阪府）
  • Year and Date: 2014-11-04 – 2014-11-06
• [Presentation] 分子混雑環境における蛋白質のNMR緩和解析 (2014)
  • Author(s): 岡村英保，木川隆則
  • Organizer: 日本生物物理学会第５２回年会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
  • Year and Date: 2014-09-25 – 2014-09-27
  • Invited
• [Presentation] 分子混雑環境における蛋白質のNMR緩和解析 (2014)
  • Author(s): 岡村英保，栃尾尚哉，杉山修世，渡部暁，フェイグマイケル，杉田有治，木川隆則
  • Organizer: 第１４回日本蛋白質科学会年会
  • Place of Presentation: 横浜産貿ホール（神奈川県）
  • Year and Date: 2014-06-25 – 2014-06-27
• [Presentation] NMRを用いたバイオマス生分解過程の追跡および解析法の開発 (2014)
  • Author(s): 西村裕志, 田頭英朗, 岡村英保, 片平正人, 渡辺隆司
  • Organizer: 第９回バイオマス科学会議
  • Place of Presentation: 高知県立県民文化ホール(高知県)
• [Presentation] 木質バイオマスを溶液NMRで解析する為の方法論の開発とその応用 (2013)
  • Author(s): 岡村英保, 西村裕志, 今村良教, 小澤佑, 寺島典二, 松下泰幸, 福島和彦, 渡部隆司, 片平正人
  • Organizer: 第52回NMR討論会
  • Place of Presentation: 石川県立音楽堂(石川県)
• [Presentation] 分子混雑環境における蛋白質のNMR緩和解析 (2013)
  • Author(s): 岡村英保, 栃尾尚哉, 杉山修世, 渡部暁, フェイグマイケル, 杉田有治, 木川隆則
  • Organizer: 第52回NMR討論会
  • Place of Presentation: 石川県立音楽堂(石川県)