Detection of gamma H2AX foci in mouse normal brain and brain tumor after boron neutron capture therapy

• Project/Area Number: 25870382
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science; Neurosurgery
• Research Institution: Kyoto University
• Principal Investigator: Kondo Natsuko 京都大学, 原子炉実験所, 助教 (00582131)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ホウ素中性子捕捉療法 / DNA二本鎖切断 / γH2AX / 悪性グリオーマ / 中性子捕捉療法 / 正常脳 / グリオブラストーマ / 脳腫瘍

Outline of Final Research Achievements

Boron neutron capture therapy (BNCT) is a particle radiation therapy in combination of thermal neutron irradiation and boron compound that specifically accumulates in the tumor. 10B captures neutrons and produces an alpha particle (He) and lithium (Li) nucleus. These particles have the characteristics of extremely high linear energy transfer (LET) radiation and therefore have marked biological effects. As the high LET radiation induces complex DNA double strand breaks (DSBs), large proportions of DSBs are considered to remain not repaired in comparison with those induced by low LET radiation. In this study, we investigated γH2AX foci as markers of DSBs in normal brain and brain tumor tissues in mouse after BNCT. γH2AX foci induced by 10B(n,α)7Li reaction remained 24 h after neutron beam irradiation. In contrast, γH2AX foci produced by γ-ray irradiation at contaminated dose in BNCT disappeared 24 h after irradiation in these tissue.

Research Products

• [Int'l Joint Research] オハイオ州立大学/脳神経外科(米国)
• [Int'l Joint Research] オハイオ州立大学/病理学(米国)
• [Journal Article] Detection of γH2AX foci in mouse normal brain and brain tumor after boron neutron capture therapy. (2016)
  • Author(s): Kondo N, Michiue H, Sakurai Y, Tanaka H, Nakagawa Y, Watanabe T, Narabayashi M, Kinashi Y, Suzuki M, Masunaga S, Ono K.
  • Journal Title: Rep Pract Oncol Radiother. Volume: 21(2) Issue: 2 Pages: 108-12
  • DOI: 10.1016/j.rpor.2014.10.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] DNA damage induced by boron neutron capture therapy is partially repaired by DNA ligase IV (2016)
  • Author(s): Kondo N, Sakurai Y, Hirota Y, Tanaka H, Watanabe T, Nakagawa Y, Narabayashi M, Kinashi Y, Miyatake S, Hasegawa M, Suzuki M, Masunaga S, Ohnishi T, Ono K.
  • Journal Title: Radiat Environ Biophys Volume: 55(1) Issue: 1 Pages: 89-94
  • DOI: 10.1007/s00411-015-0625-2
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Localized radiation necrosis model in mouse brain using proton ion beams. (2015)
  • Author(s): N.Kondo, Y.Sakurai, T.Takata, N.Takai, Y.Nakagawa, H.Tanaka, T.Watanabe, K.Kume, T.Toho, S.Miyatake, M.Suzuki, S.Masunaga and K.Ono
  • Journal Title: Appl Radiat Isot Volume: 106 Pages: 242-246
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 脳腫瘍BNCTのための生物学的基礎研究-脳壊死モデル作成と腫瘍幹細胞に関する研究- (2016)
  • Author(s): 近藤夏子、櫻井良憲、高田卓志、鈴木実、仲川洋介、田中浩基、藤本望、渡邊翼、増永慎一郎、小野公二、久米恭、宮武伸一、川端信司、高井伸彦、木村寛之、Rolf Barth、Norm Lehman、中野伊知郎
  • Organizer: 京都大学 原子炉実験所 第50回学術講演会
  • Place of Presentation: 事務棟大会議室、京都大学 原子炉実験所(大阪、泉南郡）
  • Year and Date: 2016-01-26
  • Invited
• [Presentation] 脳腫瘍BNCTのための生物学的基礎研究-脳壊死モデル作成と腫瘍幹細胞に関する研究- (2015)
  • Author(s): 近藤夏子、櫻井良憲、高田卓志、鈴木実、仲川洋介、田中浩基、藤本望、渡邊翼、増永慎一郎、小野公二、久米恭、宮武伸一、川端信司、高井伸彦、木村寛之、Rolf Barth、Norm Lehman、中野伊知郎
  • Organizer: 京都大学原子炉実験所 「BNCTの新展開－特殊な療法から一般的な療法への移行を目指して－」専門研究会
  • Place of Presentation: 事務棟大会議室、京都大学 原子炉実験所(大阪、泉南郡）
  • Year and Date: 2015-11-10
  • Invited
• [Presentation] Radiation necrosis model in mouse brain following dose escalation (2015)
  • Author(s): N.Kondo, Y.Sakurai, T.Takata, Y.Nakagawa, H.Tanaka, T.Watanabe, K.Kume, N.Takai, S.Miyatake, S.Masunaga, M.Suzuki and K.Ono
  • Organizer: 8th Young Researchers’ Boron Neutron Capture Therapy Meeting
  • Place of Presentation: Almo Collegio Borromeo, University of Pavia (Italy, Pavia)
  • Year and Date: 2015-09-13
  • Int'l Joint Research
• [Presentation] 中性子捕獲反応後組織に生じるＤＮＡ二本鎖切断のγＨ２ＡＸによる検出 (2013)
  • Author(s): 近藤夏子
  • Organizer: 中性子捕捉療法学会
  • Place of Presentation: 岡山
• [Presentation] 中性子捕獲反応後組織に生じるＤＮＡ二本鎖切断のγＨ２ＡＸによる検出 (2013)
  • Author(s): 近藤夏子
  • Organizer: 日本放射線腫瘍学会
  • Place of Presentation: 青森
• [Presentation] Detection of γH2AX foci in mouse brain tissue after neutron capture therapy (2013)
  • Author(s): Natsuko Kondo
  • Organizer: 7th Young Researchers BNCT meeting
  • Place of Presentation: Granada, Spain
• [Presentation] Detection of γH2AX foci in mouse brain tissue after neutron capture therapy (2013)
  • Author(s): Natsuko Kondo
  • Organizer: 6th Trilateral BNCT meeting between Taiwan and Japan
  • Place of Presentation: Taiawan