| Project/Area Number |
25870400
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | DNA修復 / DNA損傷 / 光生物 / 光反応 / 酵素反応機構 / 酵素 |
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| Outline of Final Research Achievements |
(6-4) photolyases are flavoproteins that are able to directly repair the (6-4) photoproducts, one of the UV-induced DNA lesions, in response to blue light in sunlight. Thus far, overall function of the (6-4) photolyase still remains obscure. Here, in order to investigate the DNA repair mechanism of the (6-4) photolyase, molecular scientific studies using mutants of the enzyme were performed. As results, several amino acids residues involved in the repair and recognition of the (6-4) photoproduct have been identified. In future, these information would be applied to the investigations on cryptochromes, which share high homologies with the (6-4) photolyase and are responsible for the regulation of the circadian clocks in human.
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