| Project/Area Number |
25870403
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
Dermatology
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| Research Institution | Osaka University |
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Principal Investigator |
OHGAKI Ryuichi 大阪大学, 医学(系)研究科(研究院), 助教 (20467525)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 表皮バリア / 脂質代謝 / リパーゼ / 皮膚生理 |
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| Outline of Final Research Achievements |
Stratum corneum lipids were extracted from both wild type- and lipase knockout neonates, and analyzed by mass-spectrometry. This lipidomics revealed that the deletion of the lipase gene alters a wide variety of lipid molecules ranging over several subclasses including ceramides, cholesterols, cholesterol esters, free fatty acids, monoglycerides and so on. Structural analyses by small angle X-ray diffraction and transmission electron microscope indicated that the lamellar lipid structure in stratum corneum was significantly disturbed whereas the overall organization of epidermal tissue was not apparently altered. These results demonstrate that the process of lipid metabolism mediated by the lipase is critically important for the maintenance of the overall lipid composition and lamellar structures of lipids in stratum corneum. The outcome of this study may contribute to identify a novel causative gene for dermatosis and to understand its pathological condition.
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