| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
|---|
| Outline of Final Research Achievements |
Neutrophils were isolated from septic patients and healthy volunteers and harvested for 4 hours. The apoptosis were assessed by Anexine V using flowcytometer, and the area of neutrophil extracellular traps (NETs) was assessed by immunofluorescence staining using anti-H1 antibody and anti-neutrophil elastase antibody. Neutrophils from healthy volunteers were stimulated by lipopolysaccharide. And the plasma cell-free DNA was assessed by realtime RT-PCR using human globin gene. Septic patients showed high cell-free DNA concentrations, low neutrophil apoptosis%, larger NETs areas. The apoptosis% of female was significantly lower than that of male in LPS-stimulated healthy volunteers’ neutrophils. NETs area of female tended to be higher than that of male in septic shock patients.
|
