Synthesis of Carbondioxide/Nitrogen Responsive Polymer Particles Bearing Switchable Recognition Capability

• Project/Area Number: 25870429
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Polymer chemistry; Chemical biology
• Research Institution: Kobe University
• Principal Investigator: Kitayama Yukiya 神戸大学, 工学(系)研究科(研究院), 助教 (40649745)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 刺激応答性 / 分子認識 / 高分子微粒子 / タンパク質 / 二酸化炭素 / 刺激応答 / 有機／無機複合粒子 / 窒素 / コロイド / 高分子合成 / 制御／リビングラジカル重合 / 金ナノ粒子

Outline of Final Research Achievements

We successfully developped the versatile CO2/N2 responsive polymer particles as described below.
(1) CO2/N2-triggered stability-controllable gold nanoparticles (AuNPs) grafted with poly(2-(diethylamino)ethyl methacrylate) (PDEAEMA) layers (PDEAEMA-g-AuNPs) were synthesized by the surface-initiated atom transfer radical polymerization of DEAEMA with AuNPs bearing initiator groups. (2) Core-shell type moleculary imprinted polymer (MIP) particles bearing protein recognition cavities in the shell layer with switchable molecular recognition capability responding to CO2 and N2 gas. (3) Effects of comonomer and polymerization temperature on the switchable molecular recognition capability of MIP particles was investigated.

Research Products

• [Journal Article] Amphiphilic Polymerizable Porphyrins Conjugated to a Polyglycerol Dendron Moiety as Functional Surfactants for Multifunctional Polymer Particle (2015)
  • Author(s): M. Moriishi, Y. Kitayama T. Ooya, T. Takeuchi
  • Journal Title: Langmuir Volume: 31 Issue: 47 Pages: 12903-12910
  • DOI: 10.1021/acs.langmuir.5b02865
  • Peer Reviewed
• [Journal Article] Synthesis of Monodispersed Submillimeter-Sized Molecularly Imprinted Particles Selective for Human Serum Albumin Using Inverse Suspension Polymerization in Water-in-Oil Emulsion Prepared Using Microfluidics (2015)
  • Author(s): Kyohei Takimoto, Eri Takano, Yukiya Kitayama, Toshifumi Takeuchi
  • Journal Title: Langmuir Volume: 31 Issue: 17 Pages: 4981-4987
  • DOI: 10.1021/acs.langmuir.5b00769
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Synthesis of CO2/N2-Triggered Reversible Stability-Controllable Poly(2-(diethylamino)ethyl methacrylate)-grafted-AuNPs by Surface-Initiated Atom Transfer Radical Polymerization (2014)
  • Author(s): Yukiya Kitayama and Toshifumi Takeuchi
  • Journal Title: Langmuir Volume: 30 Issue: 42 Pages: 12684-12689
  • DOI: 10.1021/la502801t
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Emulsifier-free, organotellurium-mediated living radical emulsion polymerization (emulsion TERP) of styrene: poly(dimethylaminoethyl methacrylate) macro-TERP (2014)
  • Author(s): Yukiya Kitayama, Masayoshi Okubo
  • Journal Title: Polymer Chemistry Volume: 5 Issue: 8 Pages: 2784-2792
  • DOI: 10.1039/c3py01539d
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Supraparticles comprised of molecularly imprinted nanoparticles and modified gold nanoparticles as a nanosensor platform (2013)
  • Author(s): Akane Uchida, Yukiya Kitayama, Eri Takano, Tooru Ooya and Toshifumi Takeuchi
  • Journal Title: RSC Advances Volume: 3 Issue: 47 Pages: 25306-25311
  • DOI: 10.1039/c3ra43660h
  • Peer Reviewed
• [Presentation] Molecularly Imprinted Nanogels for Human Serum Albumin Recognition (2015)
  • Author(s): Yukiya Kitayama, Reo Sasao, Toshifumi Takeuchi
  • Organizer: The international chemical congress of pacific basin societies 2015 (Pacifichem2015)
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] Synthesis of protein-imprinted core/shell polymer particles bearing ON/OFF switchable molecular recognition property via CO2/N2 bubbling (2015)
  • Author(s): Manabu Isomura, Yukiya Kitayama, Toshifumi Takeuchi
  • Organizer: The international chemical congress of pacific basin societies 2015 (Pacifichem2015)
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] 標的タンパク質を選択的に結合する分子インプリントナノゲル粒子の創製 (2015)
  • Author(s): 北山雄己哉•笹尾玲雄・竹内俊文
  • Organizer: 第9回バイオ関連化学シンポジウム
  • Place of Presentation: 熊本大学黒髪南地区キャンパス（熊本県・中央区）
  • Year and Date: 2015-09-10
• [Presentation] 標的タンパク質を選択的に認識する分子インプリントナノゲル粒子の合成 (2015)
  • Author(s): 北山雄己哉・笹尾玲雄・竹内俊文
  • Organizer: 第22回クロマトグラフィーシンポジウム
  • Place of Presentation: 近畿大学東大阪キャンパス（大阪府・東大阪市）
  • Year and Date: 2015-05-28
• [Presentation] Synthesis of Nanometer-sized Molecularly Imprinted Polymer Particles by Emulsifier-Free Aqueous Dispersed System in a Buffered Solution (2015)
  • Author(s): Yukiya Kitayama, Reo Sasao, Toshifumi Takeuchi
  • Organizer: 第64回高分子学会年次大会
  • Place of Presentation: 札幌コンベンションセンター(北海道・札幌)
  • Year and Date: 2015-05-27
• [Presentation] 二酸化炭素・窒素に応答して分散安定性制御可能な金ナノ粒子の合成 (2015)
  • Author(s): 北山雄己哉、竹内俊文
  • Organizer: 第64回高分子学会年次大会
  • Place of Presentation: 札幌コンベンションセンター(北海道・札幌)
  • Year and Date: 2015-05-27
• [Presentation] Molecularly Imprinted Nanoparticles as Artificial Antibodies for Protein Recognition (2015)
  • Author(s): Yukiya Kitayama, Reo Sasao, Toshifumi Takeuchi
  • Organizer: 4th International Conference on Bio-Sensing Technology
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2015-05-10
  • Int'l Joint Research
• [Presentation] Synthesis of protein-imprinted core/shell polymer particles bearing ON/OFF switchable molecular recognition property via CO2/N2 bubbling (2015)
  • Author(s): 磯村 学，北山雄己哉，竹内俊文
  • Organizer: 第95回日本化学会春季年次大会
  • Place of Presentation: 日本大学船橋キャンパス（船橋）
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] Preparation of Organic/Inorganic Hybrid Particles bearing Dispersion/Aggregation State Controllability with CO2/N2 Bubbling in Aqueous Dispersed System (2015)
  • Author(s): 北山雄己哉，竹内俊文
  • Organizer: 第95回日本化学会春季年次大会
  • Place of Presentation: 日本大学船橋キャンパス（船橋）
  • Year and Date: 2015-03-26 – 2015-03-29
• [Presentation] 表面開始原子移動ラジカル重合を用いた高分子リガンド修飾金ナノ粒子の合成とバイオマーカータンパク質の高感度検出 (2014)
  • Author(s): 北山雄己哉，竹内俊文
  • Organizer: 第63回高分子討論会
  • Place of Presentation: 長崎大学（長崎）
  • Year and Date: 2014-09-24 – 2014-09-26
• [Presentation] 二酸化炭素および窒素に応答して分散状態制御可能な有機/無機複合微粒子の合成 (2014)
  • Author(s): 北山 雄己哉，竹内 俊文
  • Organizer: 第60回 高分子研究発表会（神戸）
  • Place of Presentation: 兵庫県民会館（神戸）
  • Year and Date: 2014-07-24 – 2014-07-25
• [Presentation] 表面開始原子移動ラジカル重合による炎症マーカータンパク質高感度検出のための高分子リガンド修飾金ナノ粒子の合成 (2014)
  • Author(s): 北山 雄己哉，竹内 俊文
  • Organizer: 第60回 高分子研究発表会（神戸）
  • Place of Presentation: 兵庫県民会館（神戸）
  • Year and Date: 2014-07-24 – 2014-07-25
• [Presentation] マイクロ流路を用いたヒト血清アルブミン認識高分子微粒子の合成微粒子の合成 (2014)
  • Author(s): 瀧本 京平, 高野 恵里, 北山 雄己哉 , 竹内 俊文
  • Organizer: 第21回 クロマトグラフィーシンポジウム
  • Place of Presentation: 名古屋工業大学（名古屋）
  • Year and Date: 2014-06-05 – 2014-06-06
• [Presentation] Synthesis of Poly(methacryloyoxyethyl phosphoorylcholine)-grafted Au Nanoparticles for C-reactive Protein Sensing. (2014)
  • Author(s): Yukiya Kitayama, Toshifumi Takeuchi
  • Organizer: Pittcon2014
  • Place of Presentation: Chicago (USA)
• [Presentation] 表面開始原子移動ラジカル重合を用いたC反応性プロテイン結合性高分子リガンドを有する金ナノ粒子の合成とその選択的認識特性 (2014)
  • Author(s): 北山雄己哉、竹内俊文
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 名古屋
• [Presentation] 表面開始原子移動ラジカル重合によるPMPC 修飾金ナノ粒子の合成 (2013)
  • Author(s): 北山雄己哉、竹内俊文
  • Organizer: 第61回高分子討論会
  • Place of Presentation: 金沢
• [Presentation] 炎症マーカー認識高分子リガンド修飾金ナノ粒子の合成 (2013)
  • Author(s): 北山雄己哉、竹内俊文
  • Organizer: 第７回バイオ関連シンポジウム
  • Place of Presentation: 名古屋
• [Presentation] 分子インプリントナノ粒子ー金ナノ粒子 超分子複合体を利用した高感度センシング (2013)
  • Author(s): 内田朱音、北山雄己哉、大谷亨、竹内俊文
  • Organizer: 第62回高分子学会年次大会
  • Place of Presentation: 京都
• [Presentation] 分子インプリントナノ粒子－金ナノ粒子複合体を用いた低分子生物活性物質の高感度センシング (2013)
  • Author(s): 内田朱音、高野恵理、北山雄己哉、大谷 亨、竹内俊文
  • Organizer: 第 26回バイオメディカル分析科学シンポジウム
  • Place of Presentation: 東京