Therapeutic effect of cell transplantation with novel induced neural stem cells in stroke animal model

• Project/Area Number: 25870460
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery; General medical chemistry
• Research Institution: Okayama University
• Principal Investigator: Yamashita Toru 岡山大学, 医歯(薬)学総合研究科, 講師 (60644408)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 脳梗塞 / 細胞移植 / iN細胞 / iPS細胞 / ダイレクトリプログラミング / ﾀﾞｲﾚｸﾄﾘﾌﾟﾛｸﾞﾗﾐﾝｸﾞ / iNS細胞

Outline of Final Research Achievements

iNSC transplantation successfully improved the survival rate of stroke model mice with significant functional recovery from the stroke, suggesting that the directly converted iNSCs may represent a promising and safe cell resource for transplantation therapy in patients suffering from ischemic stroke.

Research Products

• [Journal Article] Recent Progress in Therapeutic Strategies for Ischemic Stroke. (2016)
  • Author(s): Yamashita T, Abe K.
  • Journal Title: Cell Transplant. Volume: 25 Issue: 5 Pages: 893-8
  • DOI: 10.3727/096368916x690548
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Strong improvement of apolipoprotein E/low-density lipoprotein receptor signals by telmisartan in poststroke spontaneously hypertensive stroke resistant. (2014)
  • Author(s): Yamashita T, Zhai Y, Kurata T, Hishikawa N, Morimoto N, Ohta Y, Deguchi K, Abe K.
  • Journal Title: J Stroke Cerebrovasc Dis. Volume: Oct;23(9):2240-9. Issue: 9 Pages: 2240-9
  • DOI: 10.1016/j.jstrokecerebrovasdis.2014.04.010
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Direct reprogrammed neuronal cells as a novel resource for cell transplantation therapy. (2014)
  • Author(s): Yamashita T, Abe K
  • Journal Title: Cell Transplantation Volume: Epub ahead of print
  • Peer Reviewed
• [Presentation] Direct Reprogrammed Neuronal Cells as a Novel Resource for Cell Transplantation Therapy (2015)
  • Author(s): Yamashita T, Abe K
  • Organizer: The 8th Pan Pacific Symposium on Stem Cell and Cancer Research
  • Place of Presentation: Hsinchu, Taiwan
  • Year and Date: 2015-04-10
  • Int'l Joint Research
• [Presentation] NVUと神経変性疾患 (2014)
  • Author(s): 山下徹、阿部康二
  • Organizer: 第24回脳血管シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2014-09-13
  • Invited
• [Presentation] 脳梗塞治療UpToDate ～再生医療実現への展望を含めて～ (2014)
  • Author(s): 山下徹、阿部康二
  • Organizer: 第125回日本薬理学会近畿部会
  • Place of Presentation: 岡山
  • Year and Date: 2014-06-20
  • Invited
• [Presentation] 次世代細胞（iN細胞）を用いた病態解析と再生治療への展望 (2014)
  • Author(s): 山下徹、阿部康二
  • Organizer: 第123回日本補綴歯科学会
  • Place of Presentation: 仙台
  • Year and Date: 2014-05-24
  • Invited
• [Presentation] 骨髄間葉系幹細胞とiN細胞を用いた再生医療の開発 (2014)
  • Author(s): 山下徹、阿部康二
  • Organizer: 第55回日本神経学会学術大会
  • Place of Presentation: 博多
  • Year and Date: 2014-05-21 – 2014-05-24
  • Invited
• [Presentation] In vivo optical imaging in post-stroke murine model treated by intravenous bone marrow stromal cells or free radical scavengers (2014)
  • Author(s): Yamashita T, Tian FF, Deguchi K, Omote Y, and Abe K.
  • Organizer: The 7th Pan Pacific Symposium on Stem Cell and Cancer Research
  • Place of Presentation: Taichung, Taiwan
  • Year and Date: 2014-04-12 – 2014-04-14
• [Presentation] iN cell models of Alzheimer’s disease (2014)
  • Author(s): Yamashita T, Abeliovich A, and Abe K
  • Organizer: 2nd Asian Clinical Conference
  • Place of Presentation: Kyoto, JAPAN
  • Year and Date: 2014-04-03 – 2014-04-05
• [Presentation] iN cell models of Alzheimer`s disease. (2013)
  • Author(s): Yamashita T, Abeliovich A, and Abe K
  • Organizer: The 6th Pan Pacific Symposium on Stem Cell and Cancer Research
  • Place of Presentation: Hsinchu, Taiwan
• [Presentation] Direct reprogramming from human fibroblasts to hiN cells; A novel method preparing cell resource for stroke therapy. (2013)
  • Author(s): Yamashita T, Abeliovich A, and Abe K
  • Organizer: Brain 2013
  • Place of Presentation: Shanghai, China
• [Presentation] Human induced neuronal (iN) cells; A novel method preparing cell resource for stroke therapy. (2013)
  • Author(s): Yamashita T, Abeliovich A, and Abe K
  • Organizer: Asia Pacific Stroke Conference 2013
  • Place of Presentation: Hong Kong, China
• [Presentation] iN細胞を用いた新規脳梗塞治療法開発への展望. (2013)
  • Author(s): 山下 徹、アベリオビッチ アサ、阿部康二
  • Organizer: 第25回日本脳循環代謝学会総会
  • Place of Presentation: 札幌
  • Invited