Identification and functional analysis of novel vertebrate chromosomal proteins with using Multi-Classifier Combinational Proteomics
Project/Area Number |
25870487
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Cell biology
Molecular biology
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Research Institution | Kochi University |
Principal Investigator |
Ohta Shinya 高知大学, 教育研究部医療学系基礎医学部門, 特任助教 (00631194)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 染色体 / 分裂期 / プロテオミクス |
Outline of Final Research Achievements |
In this study we identified CENP-32. CENP-32 appears to be required for centrosomes to integrate into a fully functional spindle that notonly nucleates astral microtubules, but also is able to nucleate and bind to kinetochore andcentral spindle microtubules. Additional data suggest that NuMA tethers microtubules at the anastral spindle poles and that augmin is required for centrosome detachment after CENP-32 depletion, possibly due to an imbalance of forces within the spindle. Next we identified CSAP and proposed that CSAP associates with MTs around centrosomes to stabilize MTs during mitosis, ensuring proper bipolar spindle formation and maintenance.
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Report
(4 results)
Research Products
(12 results)
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[Presentation] 多次元プロテオミクスによる分裂期染色体の制御構造解析2013
Author(s)
太田 信哉, Luis Fernando Montaño, Laura Wood, Itaru, Samejima, Flavia de Lima Alves, Juri Rapsilber, William C Earnshaw
Organizer
第64回日本染色体学会年会
Place of Presentation
富山大学(富山)
Related Report
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