| Project/Area Number |
25870505
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
Periodontology
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| Research Institution | Kyushu University |
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Principal Investigator |
MIURA Mayumi 九州大学, 大学病院, 研究員 (00404054)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | グラム陰性菌 / LPS-DNA複合体 / 歯周炎 / A. actinomycetemcomitans |
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| Outline of Final Research Achievements |
This study aimed to extract LPS-DNA complex from periodontal bacteria, and to analyze the pathogenicity. We succeeded in extracting DNA-LPS complex by using neutral phenol. It also cleared that bonding mode of LPS-DNA complex was both ionic and non-ionic bond. In addition, it was suggested that structure of the LPS affected to form LPS-DNA comlplex. Furthermore, comparing with the high-purity LPS, inflammatory cytokine TNF-α production by LPS-DNA complex was lower value. Further analysis is necessary for toxic expression of LPS-DNA complex.
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