Elucidation of transcription regulation mechanism by Pura and novel histone chaperone GRWD1
| Project/Area Number |
25870509
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
Biological pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
SUGIMOTO Nozomi 九州大学, 薬学研究科(研究院), 助教 (00633108)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | GRWD1 / Pura / p53 / 次世代シーケンサー / Purα / ChIP / 転写 |
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| Outline of Final Research Achievements |
GRWD1 is a novel histone chaperone that promotes MCM loading and may integrate regulations of cell cycle progression. We identified transcription factor Pura as a putative GRWD1 binding factor. The aim of this study was to investigate the biological relevance between Pura and GRWD1. We found that GRWD1 and Pura form a complex with p53 in vivo and negatively regulate p53 transcriptional activity. GRWD1 is overexpressed in several cancer cells. Therefore, elucidation of the p53 regulation mechanism(s) by GRWD1 and Pura may help to understand novel molecular mechanisms for induction of chromosomal instability and carcinogenesis.
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Report
(3 results)
Research Products
(6 results)
