Elucidation of transcription regulation mechanism by Pura and novel histone chaperone GRWD1

• Project/Area Number: 25870509
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology; Biological pharmacy
• Research Institution: Kyushu University
• Principal Investigator: SUGIMOTO Nozomi 九州大学, 薬学研究科(研究院), 助教 (00633108)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: GRWD1 / Pura / p53 / 次世代シーケンサー / Purα / ChIP / 転写

Outline of Final Research Achievements

GRWD1 is a novel histone chaperone that promotes MCM loading and may integrate regulations of cell cycle progression. We identified transcription factor Pura as a putative GRWD1 binding factor. The aim of this study was to investigate the biological relevance between Pura and GRWD1. We found that GRWD1 and Pura form a complex with p53 in vivo and negatively regulate p53 transcriptional activity. GRWD1 is overexpressed in several cancer cells. Therefore, elucidation of the p53 regulation mechanism(s) by GRWD1 and Pura may help to understand novel molecular mechanisms for induction of chromosomal instability and carcinogenesis.

Research Products

• [Journal Article] ATM regulates Cdt1 stability during the unperturbed S phase to prevent re-replication. (2014)
  • Author(s): Iwahori S, Kohmon D, Kobayashi J, Tani Y, Yugawa T, Komatsu K, Kiyono T, Sugimoto N, Fujita M.
  • Journal Title: Cell Cycle Volume: 13 Issue: 3 Pages: 471-481
  • DOI: 10.4161/cc.27274
  • Peer Reviewed / Open Access
• [Presentation] 新規ヒストンシャペロンGRWD1はゲノムワイ ドにクロマチン制御を行い複製ライセンシングを促進す る (2014)
  • Author(s): 杉本のぞみ et al.
  • Organizer: 第37会日本分子生物学会年会
  • Place of Presentation: 神奈川県横浜市 (パシフィコ横浜)
  • Year and Date: 2014-11-25 – 2014-11-27
  • Invited
• [Presentation] Regulation of p53 function by GRWD1, a novel histone chaperon. (2014)
  • Author(s): 都地 崇拡 et al.
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 神奈川県横浜市 (パシフィコ横浜)
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Cdt1結合蛋白質GRWD1はクロマチン構造およびMCMローディングを制御する新規ヒストンシャペロンである (2013)
  • Author(s): 杉本のぞみ et al.
  • Organizer: 第65回細胞生物学会・シンポジウム
  • Place of Presentation: 愛知県名古屋市(ウインクあいち)
  • Invited
• [Presentation] Cdt1 binding protein GRWD1 is a novel histone chaperone that regulates chromatin structure and MCM loading (2013)
  • Author(s): Nozomi Sugimoto, et al.
  • Organizer: Cold Spring Harbor Laboratory Meeting on "Eukaryotic DNA replication & Genome Maintenance"
  • Place of Presentation: Cold Spring Harbor, NY, USA
• [Presentation] Cdt1 結合蛋白質 GRWD1 は新規ヒストンシャペロンでありクロマチン構造と MCM 結合を制御 している (2013)
  • Author(s): 杉本のぞみ et al.
  • Organizer: 第36回日本分子生物学会年会・ワークショップ
  • Place of Presentation: 兵庫県神戸市(神戸ポートアイランド)
  • Invited