Development of a clinically useful molecular diagnostic method
| Project/Area Number |
25870530
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
Human genetics
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| Research Institution | Nagasaki University |
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Principal Investigator |
UNO Naoki 長崎大学, 医歯薬学総合研究科(医学系), 助教 (60624781)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | MLPA / ライゲーション / フラグメント解析 / ヘリケース依存的増幅反応 / 肺炎球菌 / キノロン耐性遺伝子 |
|---|
| Outline of Final Research Achievements |
We uncovered a ligation-independent pathway of MLPA (Multiplex ligation-dependent probe amplification) and developed a ligation-independent probe amplification system, which can be used to obtain amplified products without both the hybridization and ligation processes. Fragment analysis revealed that the ligation-independent pathway is functional and that ligation is not responsible for the specificity of MLPA. These findings indicate that the feasibility and specificity of MLPA do not rely on ligation. Furthermore, we developed a new assay for rapid identification of quinolone antibiotic resistant Streptococcus pneumoniae by using a ligation-independent probe amplification system.
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Report
(4 results)
Research Products
(2 results)
