Purification and biochemical analysis of a novel transcription nucleotide excision repair factor UVSSA
Project/Area Number |
25870534
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Functional biochemistry
Molecular biology
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Research Institution | Nagasaki University |
Principal Investigator |
GUO Chaowan 長崎大学, 原爆後障害医療研究所, 客員研究員 (70645283)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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Keywords | ヌクレオチド除去修復 / 紫外線高感受性症候群 / UVSSA / RNAポリメラーゼII / ユビキチン化 / ヌクレオチド除去修復機構 / TC-NER / コケイン症候群 / DNA修復 |
Outline of Final Research Achievements |
Transcription-Coupled Nucleotide Excision Repair (TC-NER) resolving the transcription-blocking DNA damages from our genome. Defective in TC-NER causes two genetic diseases, Cockayne syndrome (CS) and UV-sensitive syndrome (UVSS). CS patients usually exhibit photosensitivity, neurological degeneration and severe developmental defects, while UVSS patients are only sensitive to sunlight and display mild clinical features. In previous study, Nakazawa et al. identified the UVSSA gene as the responsible gene of UVSS, which encoded a novel TC-NER factor UVSSA that interacts with TC-NER machinery and stabilizes the CS complex. UVSSA also facilitates ubiquitination of RNA polymerase IIo that stalled at DNA damage sites. Recent reports shown that the ubiquitination may involve in regulation of TC-NER, thus we aimed to uncover the roles of UVSSA in RNA polymerase IIo ubiquitination during the initiation step of TC-NER, then provide more insights into the processing of stalled RNA polymerase.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] Preparation of monoPEGylated Cyanovirin-N’s derivative and its anti-influenza A virus bioactivity in vitro and in vivo.2015
Author(s)
Chongchao Wu, Wei Chen, Jia Chen, Bo Han, Zhou Peng, Feng Ge, Bo Wei, Mingxian Liu, Meiying Zhang, Chuiwen Qian, Zhibo Hou, Ge Liu, Chaowan Guo, Yifei Wang, Kaio Kitazato, Guoying Yu, Chunbin Zou, Sheng Xiong.
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Journal Title
Journal of Biochemistry
Volume: 157
Issue: 6
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Linker-Extended Native Cyanovirin-N Facilitates PEGylation and Potently Inhibits HIV-1 by Targeting the Glycan Ligand.2014
Author(s)
Jia Chen*, Dane Huang*, Wei Chen*, Chaowan Guo*, Bo Wei, Chongchao Wu, Zhou Peng, Jun Fan, Zhibo Hou, Yongsheng Fang, Yifei Wang, Kaio Kitazato, Guoying Yu, Chunbin Zou, Chuiwen Qian and Sheng Xiong.*equal contribution.
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Journal Title
PLOS one
Volume: 9
Issue: 1
Pages: e86455-e86455
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells2014
Author(s)
Lan Luo, Miho Kawakatsu, Chao-Wan Guo, Yoshishige Urata, Wen-Jing Huang, Haytham Ali, Hanako Doi, Yuriko Kitajima, Takayuki Tanaka, Shinji Goto, Yusuke Ono, Hong-Bo Xin, Kimikazu Hamano, and Tao-Sheng Li
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Journal Title
Scientific Reports
Volume: 4
Issue: 1
Pages: 1-7
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Malfunction of Nuclease ERCC1-XPF Results in Diverse Clinical Manifestations and Causes Cockayne Syndrome, Xeroderma Pigmentosum, and Fanconi Anemia.2013
Author(s)
Kashiyama, K, Nakazawa, Y, Pilz, DT, Guo, C, Sasaki, K, et al.
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Journal Title
The American Journal of Human Genetics
Volume: 92
Issue: 5
Pages: 807-819
DOI
Related Report
Peer Reviewed
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