Suppression of autoimmunity by mosue embryonic stem cell-derived dendritic cells

• Project/Area Number: 25870540
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurology; Collagenous pathology/Allergology
• Research Institution: Kumamoto University
• Principal Investigator: IKEDA TOKUNORI 熊本大学, 大学院生命科学研究部, 特任助教 (00613530)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ES細胞由来樹状細胞 / 細胞治療療法 / 自己免疫疾患 / 自己免疫疾患モデルマウス / Th1細胞 / NODマウス / EAE

Outline of Final Research Achievements

We demonstrate the immune-regulatory effect of embryonic stem cell-derived dendritic cells (ES-DCs) using two models of autoimmune disease, namely non-obese diabetic (NOD) mice and experimental autoimmune encephalomyelitis (EAE). Treatment of pre-diabetic NOD mice with ES-DCs exerted almost complete suppression of diabetes development during the observation period. Development of EAE was also inhibited by the treatment with ES-DCs, and the treatment of EAE-induced mice with ES-DCs reduced the infiltration of inflammatory cells into the spinal cord and ES-DCs suppressed Th1 cells. Moreover, the ES-DC treatment did not affect T cell response to an exogenous antigen, and we observed the inhibition of differentiation and proliferation of Th1 cells by ES-DCs in vitro. These results suggest a clinical application for pluripotent stem cell-derived DCs as a therapy for T cell-mediated autoimmune diseases.

Research Products

• [Journal Article] Suppression of Th1-mediated autoimmunity by embryonic stem cell-derived dendritic cells. (2014)
  • Author(s): 13.Suppression of Th1-mediated autoimmunity by embryonic stem cell-derived dendritic cells.da, T., Hirata, S., Takamatsu, K., Haruta, M., Tsukamoto, H., Ito, T., Uchino, M., Ando, Y., Nagafuchi, S., Nishimura, Y., Senju, S.
  • Journal Title: PLoS One Volume: 9 Issue: 12 Pages: e115198-e115198
  • DOI: 10.1371/journal.pone.0115198
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 多能性幹細胞由来樹状細胞を利用した実験的自己免疫性脳脊髄炎に対する細胞療法 (2014)
  • Author(s): 池田徳典
  • Organizer: 第55回日本神経学会学術大会
  • Place of Presentation: 福岡国際会議場 (福岡市)
  • Year and Date: 2014-05-21 – 2014-05-24
• [Presentation] ES細胞由来樹状細胞 (ES-DC)を用いた マウス自己免疫疾患モデルに対する細胞治療療法 (2014)
  • Author(s): 池田徳典
  • Organizer: 第111回日本内科学会講演会
  • Place of Presentation: 東京国際フォーラム (千代田区)
  • Year and Date: 2014-04-11 – 2014-04-13
• [Presentation] 多能性幹細胞由来樹状細胞を利用した 自己免疫疾患の細胞治療療法 (2013)
  • Author(s): 池田徳典
  • Organizer: 第41回日本臨床免疫学会総会
  • Place of Presentation: 海峡メッセ下関