Epigenetic regulation of ANGPTL2 expression in cancer and lifestyle disease
Project/Area Number |
25870543
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Pathological medical chemistry
Tumor diagnostics
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Research Institution | Kumamoto University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 癌 / 生活習慣病 / エピゲノム / 発現制御 / ANGPTL2 / 転移 |
Outline of Final Research Achievements |
We showed that the tumor microenvironment, such as hypoxia and nutrient starvation, increased ANGPTL2 expression levels in osteosarcoma cells and decreased DNA methylation levels of its promoter. In addition, the tumor microenvironment induced expression of DNA demethylation-related enzymes in osteosarcoma cells. These results suggest that the tumor microenvironment induces ANGPTL2 expression in tumor cells by inducing DNA demethylation of its promoter. We also showed that tumor cell-derived ANGPTL2 enhanced tumor metastasis through promoting invasive activity of tumor cells and tumor cell intravasation. Furthermore, We found that full-length ANGPTL2 protein was extracellularly cleaved by TLL1 into an inactive form lacking the capacity to enhance tumor aggressiveness. Thus, strategies to promote cleavage of full-length ANGPTL2 by TLL1 could block metastasis.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] ANGPTL2 increases bone metastasis of breast cancer cells through enhancing CXCR4 signaling.2015
Author(s)
Masuda T, Endo M, Yamamoto Y, Odagiri H, Kadomatsu T, Nakamura T, Tanoue H, Ito H, Yugami M, Miyata K, Morinaga J, Horiguchi H, Motokawa I, Terada K, Morioka MS, Manabe I, Iwase H, Mizuta H, Oike Y.
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Journal Title
Sci Rep.
Volume: 5
Issue: 1
Pages: 9170-9170
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Angiopoietin-like protein 2 renders colorectal cancer cells resistant to chemotherapy by activating spleen tyrosine kinase-phosphoinositide 3-kinase-dependent anti-apoptotic signaling.2014
Author(s)
Horiguchi H, Endo M, Miyamoto Y, Sakamoto Y, Odagiri H, Masuda T, Kadomatsu T, Tanoue H, Motokawa I, Terada K, Morioka MS, Manabe I, Baba H, Oike Y.
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Journal Title
Cancer Science
Volume: 105
Issue: 12
Pages: 1550-1559
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] SIRT7 controls hepatic lipid metabolism by regulating the ubiquitin-proteasome pathway.2014
Author(s)
Yoshizawa T, Karim Md. F, Sato Y, Senokuchi T, Miyata K, Fukuda T, Go C, Tasaki M, Uchimura K, Kadomatsu T, Tian Z, Smolka C, Sawa T, Kakeya M, Tomizawa K, Ando Y, Araki E, Akaike T, Braun T, Oike Y, Bober E, Yamagata K.
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Journal Title
Cell Metab.
Volume: 9
Issue: 4
Pages: 712-721
DOI
Related Report
Peer Reviewed / Open Access
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