Immunotherapy with genetically modified iPS cell-derived macrophages against melenoma
Project/Area Number |
25870545
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Dermatology
Immunology
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Research Institution | Kumamoto University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 悪性黒色腫 / 免疫療法 / 細胞治療 / iPS細胞 / マクロファージ / 腫瘍マーカー / microRNA / 腫瘍抗原 / 癌 / 免疫学 / 癌抗原 |
Outline of Final Research Achievements |
We developed immune cell therapy using induced pluripotent stem (iPS) cells. In this study, the efficacy of human iPS cell-derived macrophages (iPS-MP) genetically modified to express type I IFNs against human melanoma cells was examined. The iPS-ML expressing type I IFNs increased the expression of CD169, a marker of M1 macrophages that can activate antitumor immunity. The overexpression of type I IFNs in the iPS-ML enhanced the inhibitory effects against the disseminated human melanoma cells in SCID mice. We also found FOXM1 as a new therapeutic target of melanoma. Furthermore, we identified that hair shaft levels of microRNA-221 could be a non-invasive diagnostic tool for melanoma.
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Report
(3 results)
Research Products
(73 results)
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[Journal Article] Evaluation of sentinel node biopsy for cutaneous squamous cell carcinoma2014
Author(s)
Fukushima S, Masuguchi S, Igata T, Harada M, Aoi J, Miyashita A, Nakahara S, Inoue Y, Jinnin M, Shiraishi S, Yamashita Y, Ishihara T, Ihn H.
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Journal Title
J Dermatol.
Volume: 41
Issue: 6
Pages: 539-541
DOI
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Peer Reviewed / Acknowledgement Compliant
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[Journal Article] The expression profile of the toll-like receptor family in scleroderma dermal fibroblasts.2014
Author(s)
Sakoguchi A, Nakayama W, Jinnin M, Wang Z, Yamane K, Aoi J, Makino K, Kajihara I, Ichihara A, Makino T, Fukushima S, Sakai K, Inoue Y, Ihn H.
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Journal Title
Clin Exp Rheumatol.
Volume: 32
Pages: 4-9
Related Report
Peer Reviewed
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[Journal Article] Knockout of endothelial cell-derived endothelin-1 attenuates skin fibrosis but accelerates cutaneous wound healing.2014
Author(s)
Makino K, Jinnin M, Aoi J, Kajihara I, Makino T, Fukushima S, Sakai K, Nakayama K, Emoto N, Yanagisawa M, Ihn H.
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Journal Title
PLoS One.
Volume: 22
Issue: 5
Pages: e97972-e97972
DOI
Related Report
Peer Reviewed
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[Journal Article] Increased CCL22 expression in psoriatic skin predicts a good response to infliximab therapy.2014
Author(s)
Kusumoto S, Kajihara I, Nagamoto E, Makino K, Ichihara A, Aoi J, Johno T, Makino T, Fukushima S, Jinnin M, Ihn H.
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Journal Title
Br J Dermatol.
Volume: 171
Issue: 5
Pages: 1259-61
DOI
Related Report
Peer Reviewed
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[Journal Article] Decreased interleukin-20 expression in scleroderma skin contributes to cutaneous fibrosis.2014
Author(s)
Kudo H, Jinnin M, Asano Y, Trojanowska M, Nakayama W, Inoue K, Honda N, Kajihara I, Makino K, Fukushima S, Ihn H.
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Journal Title
Arthritis Rheumatol.
Volume: 66
Issue: 6
Pages: 1636-1647
DOI
Related Report
Peer Reviewed
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[Journal Article] Upregulation of miR-18a-5p contributes to epidermal necrolysis in severe drug eruptions.2014
Author(s)
Ichihara A, Wang Z, Jinnin M, Izuno Y, Shimozono N, Yamane K, Fujisawa A, Moriya C, Fukushima S, Inoue Y, Ihn H.
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Journal Title
J Allergy Clin Immunol.
Volume: 133
Issue: 4
Pages: 1065-74
DOI
Related Report
Peer Reviewed
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[Journal Article] Sensitive detection of melanoma metastasis utilizing circulating microRNA expression profiles2014
Author(s)
Sensitive detection of melanoma metastasis using circulating microRNA expression profiles. Shiiyama R, Fukushima S, Jinnin M, Yamashita J, Miyashita A, Nakahara S, Kogi A, Aoi J, Masuguchi S, Inoue Y, Ihn H.
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Journal Title
Melanoma Res
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Decreased IL-20 expression in scleroderma skin contributes to cutaneous fibrosis.2014
Author(s)
Kudo H, Jinnin M, Asano Y, Trojanowska M, Nakayama W, Inoue K, Honda N, Kajihara I, Makino K, Fukushima S, Ihn H.
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Journal Title
Arthritis Rheumatol
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Up-regulation of miR-18a contributes to the epidermal necrolysis in severe drug eruptions.2014
Author(s)
Ichihara A, Wang Z, Jinnin M, Izono Y, Shimozono N, Yamane K, Fujisawa A, Moriya C, Fukushima S, Inoue Y, Ihn H.
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Journal Title
J Allergy Clin Immunol
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Angiopoietin-like protein 2 accelerates carcinogenesis by activating chronic inflammation and oxidative stress2014
Author(s)
Aoi J, Endo M, Kadomatsu T, Miyata K, Ogata A, Horiguchi H, Odagiri H, Masuda T, Fukushima S, Jinnin M, Hirakawa S, Sawa T, Akaike T, Ihn H and Oike Y
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Journal Title
Mol. Cancer Res
Volume: 12
Issue: 2
Pages: 239-249
DOI
Related Report
Peer Reviewed
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[Journal Article] Proportion of Lymphocytic Inflammation with CD123 Positive Cells in Lupus Erythematous Profundus Predict a Clinical Response to Treatment.2013
Author(s)
Miyashita A, Fukushima S, Makino T, Yoshino Y, Yamashita J, Honda N, Aoi J, Ichihara A, Jinnin M, Inoue Y, Ihn H.
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Journal Title
Acta Dermato-Venereologica
Volume: 17
Issue: 5
Pages: 563-567
DOI
Related Report
Peer Reviewed
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[Journal Article] The rs2910164 G>C polymorphism in microRNA-146a is associated with the incidence of malignant melanoma.2013
Author(s)
Yamashita J, Iwakiri T, Fukushima S, Jinnin M, Miyashita A, Hamasaki T, Makino T, Aoi J, Masuguchi S, Inoue Y, Ihn H.
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Journal Title
Melanoma Res
Volume: 23
Pages: 13-20
Related Report
Peer Reviewed
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[Journal Article] Hair miR-29a levels are decreased in patients with scleroderma.2013
Author(s)
Takemoto R, Jinnin M, Wang Z, Kudo H, Inoue K, Nakayama W, Ichihara A, Igata T, Kajihara I, Fukushima S, Ihn H.
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Journal Title
Exp Dermatol
Volume: 22
Issue: 12
Pages: 832-3
DOI
Related Report
Peer Reviewed
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[Journal Article] Detection of hair-microRNAs as the novel potent biomarker: Evaluation of the usefulness for the diagnosis of scleroderma.2013
Author(s)
Wang Z, Jinnin M, Kudo H, Inoue K, Nakayama W, Honda N, Makino K, Kajihara I, Fukushima S, Inoue Y, Ihn H.
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Journal Title
J Dermatol Sci
Volume: 72
Issue: 2
Pages: 134-41
DOI
Related Report
Peer Reviewed
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[Journal Article] Serum miR-21 levels in patients with dermatomyositis.2013
Author(s)
Shimada S, Jinnin M, Ogata A, Makino T, Kajihara I, Makino K, Honda N, Nakayama W, Inoue K, Fukushima S, Ihn H.
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Journal Title
Clin Exp Rheumatol
Volume: 31
Pages: 161-2
Related Report
Peer Reviewed
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[Journal Article] The downregulation of microRNA let-7a contributes to the excessive expression of type I collagen in systemic and localized scleroderma.2013
Author(s)
Makino K, Jinnin M, Hirano A, Yamane K, Eto M, Kusano T, Honda N, Kajihara I, Makino T, Sakai K, Masuguchi S, Fukushima S, Ihn H.
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Journal Title
Journal of Immunology
Volume: 190
Issue: 8
Pages: 3905-3915
DOI
Related Report
Peer Reviewed
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[Presentation] Serum leptin receptor as a tumor marker of melanoma2014
Author(s)
Satoshi Fukushima, Hironori Mizutani, Azusa Miyashita, Junji Yamashita, Satoshi Nakahara, Masatoshi Jinnin, Hironobu Ihn
Organizer
The 39th Annual meeting of the Japanese Society for Investigative Dermatology
Place of Presentation
阪急エキスポパーク、大阪府吹田市
Year and Date
2014-12-12 – 2014-12-14
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[Presentation] Clinical significance of CD169-positive lymph node macrophages in human malignant tumors.2014
Author(s)
Koji Ohnishi, Yoichi Saito, Yoshihiro Komohara, Yuji Miyamoto, Satoshi Fukushima, Munekage Yamaguchi, Hideo Baba, Hironobu Ihn, Hidetaka Katabuchi, Motohiro Takeya
Organizer
ASCO 2014
Place of Presentation
McCormick Place, Chicago, Illinois
Year and Date
2014-05-30 – 2014-06-03
Related Report
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[Presentation] The rs2910164 G>C polymorphism in microRNA-146a is associated with the pathogenesis of malignant melanoma2013
Author(s)
Satoshi Fukushima, Junji Yammashita, Takuma Iwakiri, Masatoshi Jinnin, Azusa Miyashita, Jun Aoi, Hisashi Kanemaru, Katsunari Makino, Asako Ichihara, Yuji Inoue, Hironobu Ihn
Organizer
International Investigative Dermatology 2013,
Place of Presentation
Edinburgh, Scotland
Related Report
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