Development of therapies targeting tumor stem cells,and the genetic analysis of brain tumor usig hypoxia PET.

• Project/Area Number: 25870560
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery; Tumor diagnostics
• Research Institution: Oita University
• Principal Investigator: YASUTOMO MOMII 大分大学, 医学部, 助教 (20534192)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 悪性脳腫瘍 / メチオニンPET / 低酸素PET / 腫瘍幹細胞 / 低酸素 / PET

Outline of Final Research Achievements

The study conducted a hypoxial PET (positron emission tomography) and obtained tissue from a region diagnosed as a hypoxic region in glioblastoma tissue and from other regions. When the study cultured each tumor tissue and analyzed a factor related to oxidant stress such as HIF-1α with RT-PCR, it evidently found a influence by hypoxia in vivo.
The study compered responsiveness of anticancer drug between tumor in hypoxic region and other regions, but there was no significant difference. However, it was suggested that there was a difference in expression level of gene repair enzyme at each region and also a possible difference in effectiveness of anticancer drug under hypoxic stress.