Analysis of oral cancer microenvironment and invasion

• Project/Area Number: 25870601
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry; Pathobiological dentistry/Dental radiology
• Research Institution: Yokohama City University
• Principal Investigator: ADACHI Makoto 横浜市立大学, 医学(系)研究科(研究院), 客員研究員 (10468192)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 低酸素 / EMT / 上皮間葉移行

Outline of Final Research Achievements

The purpose of this study was to identify the role of tumor microenvironment in epithelial transformation of oral cancer cells under the hypoxic condition. Cell mortility and expression pattern of EMT-related proteins such as TWIST, Snail, and Slug in oral cancer cells were analyzed when these cells were cultured with or without CD11b+ myeloid cells and endothelial cells that are composed of tumor microenvironment. We found that oral cancer cells showed higher mortality in hypoxic condition than normoxia.