| Project/Area Number |
25870604
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
Applied microbiology
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| Research Institution | Ishikawa Prefectural University |
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Principal Investigator |
KOYANAGI Takashi 石川県立大学, 生物資源環境学部, 准教授 (20535041)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Pseudomonas 属 / 芳香族アミノ酸脱炭酸酵素 / ドーパ脱炭酸酵素 / ビタミンB6酵素 / アレロケミカル / アレロパシー / 根圏細菌 / Pseudomonas属 |
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| Outline of Final Research Achievements |
Aromatic amino acid L-DOPA acts as an allelochemical in rhizosphere to cause the growth inhibition of other organisms. In this study, we investigated the role of a rhizospheric bacterium Pseudomonas putida for plant-microbe interaction via control of the concentration of L-DOPA. P. putida harbors aromatic L-amino acid decarboxylase (AADC) which specifically catalyzes the reaction converting L-DOPA into dopamine, through this reaction the allelopathy of L-DOPA is reduced. DNA microarray analysis revealed that only the expression of the AADC structural gene was drastically induced in the presence of L-DOPA, indicating the specific role of this bacterium through responding to L-DOPA. In addition, we obtained the candidate ORFs with unknown functions possibly encoding the specific factors regulating the AADC induction. Based on these results, we could expand the grasp of AADC-mediated symbiosis between P. putida and plant.
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